2019年9月6日 · When compared to mock-treated cells expressing endogenous IDUA levels, YFP-high cells secreted 250 and 25-fold more enzyme for the SFFV and PGK-driven cassettes respectively, while cell lysates...

In primary cortical neurons, constructs using the ubiquitin C promoter directed the highest level of transgene expression; the phosphoglycerate kinase (PGK) promoter also directed robust transgene expression, while the cytomegalovirus (CMV) and MND (a synthetic promoter that contains the U3 region of a modified MoMuLV LTR with ...

2015年3月11日 · The targeting construct shown below consists of a vector backbone (vec), a 4.1 kb 5′ homology region, an YFP-encoding cassette (YFP), a phosphoglycerate kinase promoter (PGK)-driven neomycin...

1989年11月25日 · The upstream activator (UAS) of the yeast PGK gene comprises three different sequence elements. These are 1) a region of strong protein binding called the YFP, 2) three repeats of the motif CTTCC and 3) an essential activator core (AC) sequence that …

The murine phosphoglycerate kinase (PGK) promoter driving reporter gene expression allows for long term stable expression in vitro and in vivo in most types. Application Cloning in a gene: This plasmid has been designed to be compatible with a range of cloning techniques.

YFP Vector Set plasmid vectors for molecular cloning; Synonyms: cloning vector,expression vector,molecular cloning vector,plasmid,plasmid vector,vector at Sigma-Aldrich ... OGS611 PSF-CMV-PGK-KRYFP - YFP MAMMALIAN PGK PROMOTER PLASMID, plasmid vector for molecular cloning SDS Pricing; OGS513 PSF-CMV-UB-KRYFP ...

1990年9月25日 · The UAS of the yeast gene encoding the glycolytic enzyme phosphoglycerate kinase (PGK) contains several different sequence elements involved in transcriptional activation. These elements include the activator core sequence, which is bound by the RAP1 protein, and three copies of the pentamer sequenc …

This plasmid vector contains the CMV promoter upstream of the MCS and phosphoglycerate kinase (PGK) promoter driving expression of Kringle YFP (Yellow Fluorescence Protein) Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression.

The FLAG-HA-T6B-YFP DNA insert was generated by PCR using the plasmid pIRES-Neo-FLAG-HA-T6B-YFP as a template. Next, the modified pgk-ATG-FRT, was cut with NcoI, filled-in to produce blunt ends, dephosphorylated and ligated to the PCR-generated CMV-TRE-FLAG-HA-T6B-YFP DNA fragment.

2019年9月6日 · Here, we present an efficient ex vivo genome editing approach using CRISPR-Cas9 that targets the lysosomal enzyme iduronidase to the CCR5 safe harbor locus in human CD34+ hematopoietic stem and...