【求助】Western-blot ZO-1跑蛋白 - 丁香园论坛 - DXY.cn
ZO-1是膜蛋白，收蛋白时，细胞应该细胞刮刮取，而不能用胰酶消化； 至于WB条件，建议您: 1, 10%胶，电泳时，先80v 30-40min, 再120v跑，总时间1.5h左右，buffer距胶底部约2cm时止。
ZO-1 antibody (21773-1-AP) | Proteintech - ptglab
21773-1-AP targets ZO-1 in WB, IHC, IF/ICC, IF-Fro, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat, canine, hamster samples. PBS with …
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ZO-1 Polyclonal Antibody (61-7300) - Thermo Fisher Scientific
Tested in Western Blot (WB), Immunocytochemistry (ICC/IF), Immunohistochemistry (IHC), Immunoprecipitation (IP) and ELISA (ELISA) applications. This antibody reacts with Canine, …
ZO1 tight junction protein (TJP1) - Abcam中文官网
TJP1（ZO1 tight junction蛋白）、TJP2和TJP3是密切相关的支架蛋白，可以将紧密连接（Tight junction, TJ）跨膜蛋白连接到肌动蛋白细胞骨架。 紧密连接的作用是限制物质通过细胞旁间隙 …
求教如何跑出大分子蛋白zo-1 - 丁香园论坛 - DXY.cn
请问WB实验如何跑出好看的zo-1条带？ 我的是大鼠结肠组织，9个组，用的是15孔梳，用过proteintech和affinity的一抗，跑的都很丑很拉胯，拖尾重影。 我转模试过100V，140min/3
ZO-1 Antibody - Cell Signaling Technology
Polyclonal Antibody for studying ZO-1. Validated for WB. Highly specific and rigorously validated in-house, ZO-1 Antibody (CST #5406) is ready to ship.
【求助】ZO-1蛋白的western blot 检测 - 经验共享 - 分析测试百科 …
我感觉zo-1蛋白丰度低，提取时是否需要特殊的提取方法，不知道楼上是怎么提蛋白的。 最近要开始做western blot ，关于ZO-1蛋白的，分子量225kd，不知道有没有谁做过这个蛋白质，请教 …
【求助】大分子量蛋白ZO-1的western blot问题 - 丁香园论坛
做大鼠心肌的ZO-1蛋白，WB怎么也跑不出，每次都是深背景，无条带，用的湿转300、350、400mA转2h、2.5h、3h都试过，marker都能转上去，就是结果没有条带。 一抗用的life的，推 …
ZO-1 Antibody (R40.76) | SCBT - Santa Cruz Biotechnology
ZO-1 Antibody (R40.76) is a rat monoclonal IgG2a antibody designed to detect the Zonula Occludens-1 protein, also known as Tight Junction Protein 1 (TJP1), in mouse, rat, and human …
ZO 1 Antibody | Affinity Biosciences - affbiotech.com
ZO 1 Antibody - Figure 4 1-trifluoromethoxyphenyl-3-(1-propionylpiperidin-4-yl) urea (TPPU) recovered expression of tight junction proteins down-regulated by permanent cerebral middle …