2019年9月13日 · To our best knowledge, we introduce a new generation nucleic acid “ZNA” probe based biosensor for the first time in this present work. It was developed for the detection of single nucleotide mutation related to FV Leiden in combination with …

Zip Nucleic Acids (ZNA®) are a novel class of modified oligonucleotides enhancing hybridization properties of nucleic acids. ZNA® are oligonucleotides conjugated to cationic units. ZNA® show increased affinity for their targets by reducing the electrostatic repulsion …

Despite sharing similar properties to LNA and MGB in terms of higher target affinity (increased Tm), ZNA® has unique features, such as higher Tm for short probes, which enhances discrimination (SNP genotyping, miRNA detection) and can be used to reduce the cycling time or improve the accuracy of quantification of low abundant transcripts, as ...

2020年10月6日 · Objective: To investigate the performance of ZNA(ZIP Nucleic Acid) probes and its application in the quantitative detection of Chlamydia trachomatis (CT)nucleic acid. Methods: Use CT positive plasmids to compare the PCR amplification curves of …

2019年6月1日 · Zip nucleic acids (ZNAs) are new generation nucleic acids conjugated with repeated cationic spermine units that decrease electrostatic repulsions with the target nucleic acid strands. The spermine units increase their affinity to its target by decreasing electrostatic repulsion between negatively charged target DNA and probe itself.

Objective:To investigate the performance of ZNA(ZIP Nucleic Acid) probes and its application in the quantitative detection of Chlamydia trachomatis (CT)nucleic acid. Methods:Use CT positive plasmids to compare the PCR amplification curves of …

探讨ZNA （ZIP Nucleic Acid）探针的PCR性能及其在沙眼衣原体（CT）核酸定量检测中的研究。 使用CT阳性质粒，比较偶联不同ZIP数量的ZNA探针的PCR扩增曲线差异；比较ZNA探针与29mer普通Taqman探针（DNA长探针）、20mer普通Taqman探针（DNA短探针）、MGB探针在PCR扩增曲线、反复冻融稳定性及低浓度质粒检出率的差异；使用CT阳性临床样本，比较ZNA探针与DNA长探针、DNA短探针、MGB探针扩增曲线差异及低浓度样本检出率的差异。 （1）偶 …

ZNA® (Figure 1) stands for Zip Nucleic Acid, which consist in oligonucleotides conjugated with cationic spermine units. This asset decreases the electrostatic repulsion between single strand nucleic acids (i.e. between primer/probe and target DNA during annealing).

2019年9月13日 · In the present study, an impedimetric biosensing platform based on zip nucleic acids (ZNA) was developed for the sensitive detection of Factor V Leiden (FV Leiden) mutation.

ZNA®s enable specific and sensitive reactions when used as primers for PCR and Reverse Transcription (Figure 3-5). Moreover, ZNA® probes provide broad flexibility in assay design and represent an effective alternative to Minor Groove Binder (MGB)- and Locked Nucleic Acid (LNA)-containing oligonucleotides (3; 4).