Zip Nucleic Acids (ZNA®) are a novel class of modified oligonucleotides enhancing hybridization properties of nucleic acids. ZNA® are oligonucleotides conjugated to cationic units. ZNA® show increased affinity for their targets by reducing the electrostatic repulsion …

Despite sharing similar properties to LNA and MGB in terms of higher target affinity (increased Tm), ZNA® has unique features, such as higher Tm for short probes, which enhances discrimination (SNP genotyping, miRNA detection) and can be used to reduce the cycling time or improve the accuracy of quantification of low abundant transcripts, as ...

The Tm of ZNAs® increases linearly with the length of the oligocation. Therefore, it is possible to easily predict the melting temperature (Tm) of ZNA®-DNA or ZNA®-RNA hybrids. Figure 1. Structure of one single 5'-ZNA (R = sequence). metabion offers …

ZNA (TM) (Zip Nucleic Acids) are oligocation-oligonucleotide conjugates that demonstrate an increased affinity for their complementary sequence without reduction in specificity. Increased affinity derives from the molecules’ cationic moieties (functional groups), which reduce the charge repulsion between the two nucleic acid strands.

The possibility of modulating the global charge of the ZNA® oligonucleotide by the number of cationic spermine moieties attached to the nucleic acid oligomer is key to easily predict the melting temperature of ZNA®-DNA/ZNA®-RNA hybrids. Tm increases linearly with the length of the oligocation (Figure 2; 1).

2010年3月3日 · ZNA (TM) (Zip Nucleic Acids) are oligocation-oligonucleotide conjugates that demonstrate an increased affinity for their complementary sequence without reduction in specificity. Increased affinity derives from the molecules’ cationic moieties (functional groups), which reduce the charge repulsion between the two nucleic acid strands.

2015年9月23日 · ZNA™ (Zip Nucleic Acids) are oligocation-oligonucleotide conjugates that have an increased affinity for their complementary sequence without losing selectivity. This affinity increase is due to the cationic moieties, which reduces the charge repulsion between the two strands of nucleic acid.

ZNA® are a novel class of modified oligonucleotides enhancing hybridization properties of nucleic acids. ZNA are oligonucleotides conjugated to cationic units. Their global charge can be modulated by the number of cationic spermine moieties grafted on each oligonucleotide.

Zip Nucleic Acid (ZNA) 為一種新型核苷酸修飾物。ZNA 結購是在DNA序列外側加上帶正電荷的精胺基團(spermine unit，也稱為Z unit)，正電荷結構幫助減少ZNA與互補序列間的負電排斥力，嵌住互補序列並形成狀似拉鍊結構，如下方作用機制圖示，因此被命名為Zip Nucleic Acid。

2019年9月30日 · To attenuate the electrostatic repulsion, Zip Nucleic Acids (ZNA) was developed, consisting of oligonucleotides conjugated to positively charged spermine derivatives (Noir et al., 2008). It facilitates target recognition by allowing the oligonucleotide to scan the single stranded DNA till it finds the complementary sequence.