Here in this study, we determined the solution structure of USP20 ZnF-UBP domain and investigated its binding properties with mono-ubiquitin and poly-ubiquitin (K48-linked di-ubiquitin) by using NMR and molecular modeling techniques.

2023年10月7日 · usp16除了其usp域外，还包含一个n端锌指泛素结合域(znf-ubp)域。 为了阐明40S结合需要USP16的哪些结构域，作者生成了C端StHA标记的USP16截断结构，其中仅包含ZnF-UBP结构域或USP结构域。

2006年3月24日 · Ubiquitin binding proteins regulate the stability, function, and/or localization of ubiquitinated proteins. Here we report the crystal structures of the zinc-finger ubiquitin binding domain (ZnF UBP) from the deubiquitinating enzyme isopeptidase T (IsoT, or USP5) alone and in complex with ubiquitin.

2023年5月1日 · HDAC6 ZnF-UBP can bind to unanchored ubiquitin, facilitating protein degradation via autophagy-lysosome pathway. HDAC6 participants in diseases like neurodegeneration, cancer, and viral infections, through its non-enzymatic functions. This review appeals for the development and application of effective HDAC6 ZnF-UBP inhibitors.

2008年8月1日 · Among these enzymes, three USPs contain a zinc-finger ubiquitin-specific protease (ZnF-UBP) domain, indicating that this domain plays a crucial part in regulating their activity. To address the putative function of this domain, we systematically analysed and aligned yeast and human ZnF-UBP-containing proteins.

2019年8月9日 · 在本研究中，我们利用 NMR 和分子建模技术确定了 USP20 ZnF-UBP 结构域的溶液结构，并研究了其与单泛素和多泛素（K48 连接的双泛素）的结合特性。 USP20 的 ZnF-UBP 结构域形成一个球形折叠，由中心 β 片层组成，片层两侧各有一个 α 螺旋或两个 α 螺旋。 然而，尽管USP20 ZnF-UBP已经形成了泛素识别所必需的规范核心结构，但其泛素结合能力较弱。 通过 NMR 数据驱动的对接揭示了了解 USP20 ZnF-UBP 泛素结合能力的结构基础。

2019年7月6日 · USP20 contains four distinct structural domains including the N-terminal zinc-finger ubiquitin binding domain (ZnF-UBP), the catalytic domain (USP domain), and two tandem DUSP domains, and none of the structures for these four domains has been solved.

2006年3月24日 · Ubiquitin binding proteins regulate the stability, function, and/or localization of ubiquitinated proteins. Here we report the crystal structures of the zinc-finger ubiquitin binding domain (ZnF UBP) from the deubiquitinating enzyme isopeptidase T (IsoT, or USP5) alone and in complex with ubiquitin. …

Ubiquitin binding proteins regulate the stability, function, and/or localization of ubiquitinated proteins. Here we report the crystal structures of the zinc-finger ubiquitin binding domain (ZnF UBP) from the deubiquitinating enzyme isopeptidase T (IsoT, or …

2020年12月8日 · Here, we have studied the role of the HDAC6 ZnF UBP domain as a modifier of Tau aggregation by its direct interaction with the polyproline region/repeat region of Tau. Interaction of HDAC6 ZnF UBP with Tau was found to reduce the propensity of Tau to self-aggregate and to disaggregate preformed aggregates …