cDNA (complementary DNA)即互补DNA或拷贝DNA。 任何cDNA克隆方法的关键环节都是以mRNA为模板利用 反转录酶 (reverse transcriptase)合成cDNA的第一条链，反转录酶没有引物不能起始DNA的合成，真核生物多利用oligo (dT…

2024年6月3日 · cDNA cloning is a molecular biology technique used to isolate and amplify specific genes from an organism’s mRNA to produce complementary DNA (cDNA) and then insert it into a cloning vector for further study or expression.

2020年7月18日 · Complementary DNA (cDNA) cloning is termed for the gene cloning (cloning of DNA fragments) obtained from cDNA. The principle of cDNA cloning is that it involves the copying of mRNA transcripts into DNA, which are then inserted into bacterial plasmids and then placed into bacteria by transformation.

Find cDNA and ORF clones for Yeast, E. coli, C. elegans, D. rerio, and Xenopus. See our cDNA & ORF selection guide to learn more about each collection. Fully sequenced, pre-cloned cDNA & ORFs for rapid gene overexpression studies.

2020年8月14日 · cDNA 克隆：是全长转录本的cDNA克隆，既包含了全长蛋白质的编码序列，也包含 5' 和 3' UTR非编码序列，不能直接用于转染细胞表达，需要把5’ 及3’ UTR截去然后将表达框连接到构建好的表达载体才可以进行转染细胞并表达。

5 天之前 · cDNA (short for copy DNA; also called complementary DNA) is synthetic DNA that has been transcribed from a specific mRNA through a reaction using the enzyme reverse transcriptase

When searching the NCBI Gene Database, look for "NIH cDNA clone" hypertext links, which lead to one or more cDNA sequences. Similar links may be found on protein and UniGene entries. In the full report for a gene, look in the "Links" section on the right for possible MGC clones.

Genome-wide coverage of cDNA clones, tagged ORF clones, non-tagged native cDNA clones, are in mammalian expression vectors, also in Lentiviral vectors. The ORFs can be easily shuttled in over 100 expression vectors with different tags and selection markers. 93% of genes are in stock; same time and get your result quickly.

cDNA cloning is isolating and amplifying a single, self-replicating organism that includes within its DNA, a cDNA that is of interest to the experimenter. In some cases cDNA cloning may simply refer to the isolation of any single cDNA, since, in some circumstances, an experimentalist may be interested in any cDNA produced by a particular tissue.

Construction of cDNA clones involves the synthesis of complementary DNA from mRNA and then inserting a duplex copy of that into a cloning vector, followed by transformation of bacteria.