cDNA-AFLP技术结合了RT-PCR和AFLP技术，其基本原理：以纯化的mRNA为模板，反转录合成cDNA。 用识别序列分别为6-bp和4-bp的两种限制性内切酶酶切双链cDNA，酶切片段与人工接头连接后，利用与接头序列互补的引物进行预扩增和选择性扩增，扩增产物通过聚丙烯酰胺凝胶 ...

扩增片段长度多态性（AFLP）是1993年 荷兰 科学家Zabeau和Vos发展起来的一种检测DN A多态性的新方法。 对 基因组DNA 进行 双酶切，形成分子量大小不同的随机限制片段，再进行 …

2007年5月31日 · In essence, the cDNA-AFLP method involves reverse transcription of mRNA into double-stranded cDNA, followed by restriction digestion, ligation of specific adapters and fractionation of this...

Amplified fragment length polymorphism (AFLP) is a PCR-based technique that uses selective amplification of a subset of digested DNA fragments to generate and compare unique fingerprints for genomes of interest. The power of this method relies ...

Amplified fragment length polymorphism (AFLP-PCR or AFLP) is a PCR-based tool used in genetics research, DNA fingerprinting, and in the practice of genetic engineering.

2024年5月23日 · The cDNA-AFLP method involves the reverse transcription of mRNA into double-stranded cDNA, followed by restriction digestion, ligation of specific adapters, and fractionation of the resulting cDNA fragments into smaller subsets through selective PCR …

AFLP 是通过限制性内切酶片段的不同长度检测 DNA 多态性的一种 DNA 分子标记技术。 本 AFLP 技术主要特点 ：分析所需 DNA 量少，仅需 0.5g；可重复性好；多态性强；分 辨率高；不需要 Southern 杂交；样品适用性广…

2003年3月18日 · An improved cDNA-AFLP method for genome-wide expression analysis has been developed. We demonstrate that this method is an efficient tool for quantitative transcript profiling and a valid alternative to microarrays.

cDNA-AFLP技术是在AFLP技术基础上发展起来的主要用于研究基因差异的技术，具有高效可靠的优点，受到研究者的青睐，被广泛应用于包括植物在内的各类生物的基因表达的研究中。

The method of cDNA-AFLP allows detection of differentially expressed transcripts using PCR. This report provides a detailed and updated protocol for the cDNA-AFLP procedure and an analysis of interactions between its various parameters.