2021年5月24日 · 293T细胞中加入cAMP激动剂Forskolin (FSK)和糖皮质激素类似物地塞米松 (Dex)可显著诱导cAMP对Pck1和G6pc报告基因的转录激活 (辅图1A)。 科研人员将包含以激酶 …

Recombinant HEK293 cells stably overexpress human glucagon-like peptide-1 (GLP1R) on the surface and contain high levels of G protein Gαs to couple with the receptor in downstream …

We show that when the two receptors were co-expressed in HEK 293T cells with comparable receptor ratio to pancreatic cancer cells, GIP predominately induced cAMP accumulation while …

2018年10月1日 · Here we apply our green fluorescent cADDis sensor to the detection of Gαi-mediated inhibition of adenylyl cyclase activity. We validated and optimized the assay in living …

2021年8月26日 · We show that when the two receptors were co-expressed in HEK 293T cells with comparable receptor ratio to pancreatic cancer cells, GIP predominately induced cAMP …

2024年4月25日 · Here, we report that BRS3 and EP3 form dimers in the membrane of living HEK-293T cells. BRS3-EP3 dimers switched to couple Gα s protein upon PGE2 stimulation, which …

We validated and optimized the assay in living HEK 293T cells using several known Gαi-coupled receptors and agonists, and we report robust Z' statistics and consistent EC 50 responses. …

Method: HEK293 cells were treated with vehicle, forskolin, epinephrine, 11,12-EET, 11,12-DHET, as well as potential pathway and G-protein inhibitors to assess changes in cAMP production. …

In HEK293T cells stably transfected with RXFP1, preliminary studies confirmed the biphasic cAMP response, with an initial Galphas component and a delayed response involving PI3K …

Trusted human HEK293T cell line (KC-1837) for drug screening and biological assays. Stable 293T cell line expressing exogenous luciferase gene under the control of a CAMP response …