相比于蛋白质印迹技术， 斑点印迹分析法 (Dot Blotting)通过简化实验流程，可以 完成高通量样本蛋白质表达水平的检测，但是实验结果依然只能以“半定量”形式体现。

dsRNA是IVT副产物中一个非常重要的杂质，能够被细胞内对应的核酸受体识别，例如TLR3，MDA5或者RIG-I，引发天然免疫炎症反应。 一方面，dsRNA是一种天然佐剂。 但是，目前，并没有确定性的数据来证实dsRNA的存在为新…

Cao and colleagues develop a rapid and sensitive lateral flow strip assay (LFSA) for the detection of dsRNA contaminants in in vitro-transcribed (IVT) mRNA. This straightforward method is 16 times faster than the gold-standard dot-blot assay, while also constituting an early warning tool for the prevention of potential immunogenicity from dsRNA.

2024年6月19日 · Here we show that antisense RNAs (asRNAs) accelerate mRNA export by annealing with their sense counterparts through the helicase Dbp2. These double-stranded RNAs (dsRNAs) dominate export compared...

An antiserum against polyinosinic-polycytidylic acid (In-Cn) was used to detect double-stranded RNA (dsRNA) by several serological techniques. DsRNA was readily detected by indirect ELISA (ELISA-I) and dot immunobinding assay (DIA).

Lumit® dsRNA Detection Assay is a homogeneous, bioluminescent approach for dsRNA quantification, eliminating the need for antibodies or wash steps. This assay uses NanoLuc® Binary Technology (NanoBiT), a split luciferase complementation system specifically designed for biomolecular interaction studies.

2023年3月17日 · Characterization of double-stranded (ds)RNAs is relevant to the understanding of viral replication and immune sensing. Here, we provide a protocol describing the use of anti-dsRNA antibodies for immunofluorescence and immunoblotting in virus-infected insect cells, which can also be applied to tissues and other organisms.

5 天之前 · Dot blot法中，首先将dsRNA标准品和待检mRNA样品滴在尼龙膜上，干燥、封闭后，加入检测抗体J2，孵育1hr后，洗去未结合检抗，再加入酶标二抗，同样孵育1hr后，洗去未结合酶标二抗，加入底物反应显色…

Detection of dsRNA by Dot Blot Principle: The RNA dot blot represents a simplified version of the northern blot enabling rapid and specific identification of RNA molecules of interest. Samples are spotted onto the membrane and subjected to incubation with a …

Dot blot assays using SCICONS’s Anti-dsRNA monoclonal antibody J2 are a fast and easy method for the detection of dsRNA in both crude in vitro transcription mixes as well as purified mRNA [1-6].