在识别 AUG 后，eIF2 在GTP酶活化蛋白 eIF5 的帮助下可将 GTP 水解为 GDP，并从 mRNA 上分解下来，以结合 60S 核糖体亚基并延长多肽链。 在 eIF5 作为 GDI 而存在的情况下，eIF2 保持与 GDP 的结合。 要开始另一轮翻译，eIF2B 必须同时作为 GDI 位移因子 (GDF) 和鸟嘌呤交换因子 (GEF)，从而在 eIF2 上将 GDP 交换为 GTP。 此步骤必须受到密切调控，不同家族的 4 种应激激活的激酶 — PKR (dsRNA)、PERK（ER 应激）、GCN2（氨基酸饥饿）和 HRI（血红素不 …

Polyclonal Antibody for studying EIF2S1/eIF2-alpha (Ser52) phosphate. Validated for WB, WB. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-eIF2α (Ser51) Antibody (CST #9721) is ready to ship.

In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51.

Polyclonal Antibody for studying EIF2S1/eIF2-alpha. Validated for WB. Highly specific and rigorously validated in-house, eIF2α Antibody (CST #9722) is ready to ship.

Phosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. eIF2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation initiation by ...

The eukaryotic translation initiation factor 2 (eIF2) has key functions in the initiation step of protein synthesis. eIF2 guides the initiator tRNA to the ribosome, participates in scanning of the mRNA molecule, supports selection of the start ...

eIF2 alpha Antibody - Western blot analysis of extracts from Rat brain, using eIF2 alpha Antibody.

PKR Antibody (MA5-56942) in WB Western blot analysis of PKR. Total cell lysates (30 μg) from C2C12, H9c2, HAP-1, HeLa, HepG2, and HT-1080 cell lines were loaded and separated by SDS-PAGE. ... EIF2AK2 (PKR) is one of 4 kinases that specifically phosphorylate Ser51 of translation initiation factor eIF2-alpha in response to various environmental ...

The eIF2 initiation complex integrates a diverse array of stress-related signals to regulate both global and specific mRNA translation. Under permissive conditions, eIF2 binds GTP and Met-tRNAi to form the ternary complex (TC), which then associates with the 40S ribosomal subunit, eIF1, eIF1A, eIF5, and eIF3 to form the 43S pre-initiation ...

We discuss novel insight into the role and consequences of the phosphorylation of the translation initiation factor eIF2α in the context of stress responses and cell-cycle regulation. eIF2α is centrally located to regulate translation and its phosphorylation in response to different environmental challenges is one of the best characterized stres...