Polyclonal Antibody for studying EIF2S1/eIF2-alpha (Ser52) phosphate. Validated for WB, WB. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-eIF2α (Ser51) Antibody (CST #9721) is ready to ship.

2010年7月21日 · Various forms of stress induce pathways that converge on the phosphorylation of the alpha (α) subunit of eukaryotic translation initiation factor eIF2 at serine 51 (S51), a modification that results in a global inhibition of protein synthesis.

Western blot analysis of lysates from HeLa cells, using Phospho-eIF2α-S51 Rabbit pAb . HeLa cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight.

2015年1月15日 · We observed that immortalized mouse embryonic fibroblasts (MEFs) expressing a serine 51 to alanine (S51A) mutant of eIF2 α P (herein referred to as knock-in (KI) cells) were highly susceptible to...

2020年6月8日 · Second, the modulation of autophagy by >30 chemically unrelated agents was partially inhibited in cells expressing a non-phosphorylable (S51A) mutant of eIF2α or lacking all four eIF2α kinases,...

Phospho-eIF2α (Ser51) (D9G8) XP ® Rabbit mAb detects endogenous eIF2α only when phosphorylated at Ser51. The antibody does not recognize elF2α phosphorylated at other sites. Human eIF2alpha residue Ser52 historically has been referenced as Ser51. Human, Mouse, Rat, Monkey, D. melanogaster.

Immunohistochemical analysis of paraffin-embedded human colon cancer, using Phospho-eIF2 alpha (Ser51) Antibody. Immunofluorescent analysis using the Antibody at 1:50 dilution. …

Eukaryotic cells respond to various forms of stress by blocking mRNA translation initiation via the phosphorylation of the alpha (α) subunit of eIF2 at serine 51 (S51) (eIFαP). An important role of eIF2αP is the regulation of redox homeostasis and adaptation of cells to oxidative stress.

Immunoprecipitation analysis of 0.2mg HeLa treated with Calyculin A (100 nM) extracts using p-eIF2α-S51 antibody (IP:3ug;WB:1:1000).Control IgG: Normal Rabbit IgG. WB 1:1000-2000. IP 0.5μg-4μg. 36kDa. 1965. P05198. Rabbit. Polyclonal Antibody. IgG. Cytoplasmic granule. translational initiation. Affinity purification.