
Internal cap-initiated translation for efficient protein production ...
2025年2月19日 · Here we introduce two molecular designs that bolster circular mRNA translation through an internal cap-initiated mechanism. The first consists of a circular mRNA with a …
CleanCap M6 inhibits decapping of exogenously delivered IVT …
Protein expression of exogenous mRNA is a function of both mRNA half-life and translation efficiency. Considering that translation initiation begins with eukaryotic translation initiation …
Control of the eIF4E activity: structural insights and ... - Springer
2021年9月19日 · The central role of eukaryotic translation initiation factor 4E (eIF4E) in controlling mRNA translation has been clearly assessed in the last decades. eIF4E function is essential …
Chemical and topological design of multicapped mRNA and …
2024年9月23日 · A dual-capped oligo effectively enhanced eIF4E affinity, reducing the K d from 2,214 ± 139 nM to 1,414 ± 135 nM (Fig. 3d and Extended Data Fig. 3f,j), and such enhanced …
Interaction of trinucleotide cap analogues with eIF4E. (A ...
To this end, eIF4E protein was titrated with increasing concentrations of cap 0 and cap 1 variants of A-, G-or Ctrinucleotides and fluorescence intensity changes at 337 nm (upon 280 nm …
Nat. Biotechnol. | 戴上启动帽,MIT王潇团队提出LEGO增强翻译能力
2024年11月7日 · 结果表明,双帽寡核苷酸显著增强了eIF4E的亲和力,将解离常数(Kd)从2214 ± 139 nM降低至1414 ± 135 nM。 接下来,作者进行了原位RNA分析,以确认双帽结构确实增 …
真核起始因子4E - 维基百科,自由的百科全书
真核起始因子4E(英文:eukaryotic initiation factor 4E,简称“eIF4E”)是一种帽结合蛋白,可以特异性地识别mRNA的5'端的帽子结构,它在真核翻译的起始过程中发挥重要作用。
具有三磷酸链修饰的三核苷酸帽类似物:作为 mRNA 加帽试剂的合 …
最近的 COVID-19 大流行证明了体外转录 (IVT) mRNA 的巨大治疗潜力,但其生化特性的改进,如细胞稳定性、反应原性和翻译活性,对于基因替代疗法和抗癌免疫疗法的进一步实际应用 …
eIF4E 活性的控制:结构见解和药理学意义,Cellular and Molecular …
eIF4E 和真核起始因子 4G (eIF4G) 之间的相互作用对于翻译机器的组装至关重要,这是 mRNA 翻译的初始步骤。 一组特征明确的蛋白质,称为 4E 结合蛋白 (4E-BPs),通过竞争 eIF4E 表面 …
Trinucleotide cap analogs with triphosphate chain modifications ...
2024年9月9日 · In this study, we combined a highly efficient trinucleotide IVT capping technology with several modifications of the 5' cap triphosphate bridge to synthesize a series of 16 new …