Development of Biochemical Assays for the Identification of eIF4E ...
2012年6月1日 · This work describes the development and validation of FP- and SPR-based assays to monitor the interaction between eIF4E and the cap structure. Two probes with …
Binding preference of eIF4E for 4E-binding protein isoform and …
2007年4月13日 · The SPR analyses of the eIF4E–4E-BP pairs showed the binding preference in the order of 4E-BP2 > 4E-BP3 > 4E-BP1 regardless of the cap-free or cap-bound state of …
Identification and function of the second eIF4E-binding region in …
2011年10月28日 · In this work, we investigated the feature of the binding of eIF4G to m 7 GTP-bound eIF4E by the surface plasmon resonance (SPR) method and isothermal titration …
Binding preference of eIF4E for 4E-binding protein isoform and …
2007年4月13日 · To investigate the binding preference of eIF4E for the three eIF4E-binding isoforms (4E-BP1-3) and the function of N-terminal flexible region of eIF4E for their …
Cooperative modulation by eIF4G of eIF4E‐binding ... - The EMBO …
1998年8月17日 · Cap‐analogue chromatography and surface plasmon resonance (SPR) analyses demonstrate that one class of mutations in these surface regions disrupts eIF4E–eIF4G …
Improved eIF4E Binding Peptides by Phage Display Guided Design ...
2012年10月19日 · SPR measurements and atomistic simulations of eIF4E and its complexes with a variety of peptides reveal how the conserved motif (YXXXXLΦ) in the peptides is …
eIF4E-independent translation is largely eIF3d-dependent
2024年8月6日 · We study here how mRNAs are translated in an eIF4E1-independent manner by blocking eIF4E1 using a constitutively active version of eIF4E-binding protein (4E-BP). Via …
Combined analysis of eIF4E and 4E-binding protein expression …
Increased eukaryotic translation initiation factor 4E (eIF4E) expression occurs in many cancers, and makes fundamental contributions to carcinogenesis by stimulating the expression of …
Phosphorylation of Eukaryotic Initiation Factor 4E Markedly …
2002年2月1日 · In eukaryotes, a key step in the initiation of translation is the binding of the eukaryotic initiation factor 4E (eIF4E) to the cap structure of the mRNA. Subsequent …
Phosphorylation of eIF4E attenuates its interaction with mRNA 5′ …
2002年9月4日 · The association and dissociation rates for binding of capped mRNA to eIF4E, as roughly estimated by SPR, are at odds with the clamping model, inasmuch as an increase of …