2012年6月1日 · This work describes the development and validation of FP- and SPR-based assays to monitor the interaction between eIF4E and the cap structure. Two probes with different affinities for eIF4E were evaluated and their range of …

2007年4月13日 · The SPR analyses of the eIF4E–4E-BP pairs showed the binding preference in the order of 4E-BP2 > 4E-BP3 > 4E-BP1 regardless of the cap-free or cap-bound state of eIF4E. This preferred binding is mainly due to the differences in their dissociation rates, because no notable differences were observed among the k a values of 4E-BP1–3.

2011年10月28日 · In this work, we investigated the feature of the binding of eIF4G to m 7 GTP-bound eIF4E by the surface plasmon resonance (SPR) method and isothermal titration calorimetry (ITC), in which the eIF4GI fragment peptides including the eIF4E recognition Y (X) 4 Lφ motif (first binding site) and/or the peptide corresponding to the second eIF4E binding...

2007年4月13日 · To investigate the binding preference of eIF4E for the three eIF4E-binding isoforms (4E-BP1-3) and the function of N-terminal flexible region of eIF4E for their interactions, the binding parameters of recombinant full-length and N-terminal residues-deleted eIF4Es with 4E-BP1-3 were investigated by the surface plasmon resonance (SPR) analysis.

1998年8月17日 · Cap‐analogue chromatography and surface plasmon resonance (SPR) analyses demonstrate that one class of mutations in these surface regions disrupts eIF4E–eIF4G association, and thereby polysome formation and growth.

2012年10月19日 · SPR measurements and atomistic simulations of eIF4E and its complexes with a variety of peptides reveal how the conserved motif (YXXXXLΦ) in the peptides is responsible for binding eIF4E. The Y4 residue, as revealed by crystallography, makes a critical hydrogen bond between its hydroxyl group and the carbonyl backbone of P38 of eIF4E.

2024年8月6日 · We study here how mRNAs are translated in an eIF4E1-independent manner by blocking eIF4E1 using a constitutively active version of eIF4E-binding protein (4E-BP). Via ribosome profiling we...

Increased eukaryotic translation initiation factor 4E (eIF4E) expression occurs in many cancers, and makes fundamental contributions to carcinogenesis by stimulating the expression of cancer-related genes at post-transcriptional levels.

2002年2月1日 · In eukaryotes, a key step in the initiation of translation is the binding of the eukaryotic initiation factor 4E (eIF4E) to the cap structure of the mRNA. Subsequent recruitment of several components, including the small ribosomal subunit, is thought to allow migration of initiation complexes and recognition of the initiation codon.

2002年9月4日 · The association and dissociation rates for binding of capped mRNA to eIF4E, as roughly estimated by SPR, are at odds with the clamping model, inasmuch as an increase of the dissociation rate (10-fold) is observed upon phosphorylation of eIF4E.