2019年11月26日 · The potyvirus-derived viral genome-linked protein (VPg) is covalently bound to the 5' end of viral genomic RNA (gRNA) and associates with host eIF4E for successful infection. Divergent models to explain these observations proposed either an unknown mode of eIF4E engagement or a competition of VPg for the m 7 G cap-binding site.

2019年11月12日 · Here, we unexpectedly found that a small viral protein, viral genome-linked protein (VPg), directly binds the cap-binding site of eIF4E, indicating that eIF4E can additionally be controlled through direct competition with its cap-binding site.

2021年3月22日 · Eukaryotic translation initiation factor 4E (eIF4E) plays a key role in the infection of potyviruses in susceptible plants by interacting with viral genome-linked protein (VPg). Sugarcane ( Saccharum spp.) production is threatened by mosaic disease caused by Sugarcane mosaic virus (SCMV), Sorghum mosaic virus (SrMV), and Sugarcane streak mosaic ...

2024年11月25日 · PVY isolates carrying equivalent amino acid mutations of K105E or V108I in VPg can re-hijack eIF(iso)4E and infect eIF4E-knockdown tobacco plants (Takakura et al. 2018). These results suggested that the amino acids at positions 105 and 108 of potyviral VPg may be critical for breaking eIF4E-mediated resistance.

VPg directly bound the cap-binding site of eIF4E and competed for m7G cap analog binding. In human cells, VPg inhibited eIF4E-dependent RNA export, translation, and oncogenic transformation. Moreover, VPg formed trimeric complexes with eIF4E eIF4G, eIF4E bound VPg RNA conjugates, and.

Translation of viral RNA starts with the interaction between the plant cap-binding translation initiation factors eIF4E and viral genome-linked protein (VPg) covalently attached to the viral RNA. Disruption of this interaction is one of the natural mechanisms of plant resistance to PVY.

2019年11月11日 · To dissect these possibilities, we resolved the structure of VPg, revealing a previously unknown 3-dimensional (3D) fold, and characterized the VPg–eIF4E complex using NMR and biophysical...

We demonstrated that VPg binds the cap-binding site of eIF4E and forms trimeric complexes with eIF4E and eIF4G; furthermore, we demonstrated that VPg–RNA conjugates directly bind eIF4E and were templates for translation consistent with the requirement of potyviruses for VPg–eIF4E interactions for their lifecycle.

2021年3月22日 · 本研究揭示了甘蔗花叶病菌侵染甘蔗植物的分子机制，有利于甘蔗育种抗甘蔗花叶病。 Eukaryotic translation initiation factor 4E (eIF4E) plays a key role in the infection of potyviruses in susceptible plants by interacting with viral genome-linked protein (VPg).

With the dual objective of testing the impact of the distinct combinations of amino acid changes identified in eIF4E proteins on interaction with the viral VPg, and obtaining insights into molecular mechanisms underlying viral adaptation to eIF4E-mediated resistance (i.e. resistance breaking), we performed a systematic study of the physical ...