eIF4G is an important scaffold for the eIF4F complex and aids in recruiting the 40S ribosomal subunit to mRNA. There are three mechanisms that the 40S ribosome can come to recognize the start codon: scanning, internal entry, and shunting .

大的eIF4G亚基有eIF4E、PABP和RNA的结合位点，并能形成闭环的环化mRNA/RNA结合蛋白（RPB）复合物。 eIF4A是一种RNA螺旋酶，eIF4F的形成和附属因子eIF4B与eIF4A的结合增强了eIF4A的活性。 eIF4A解开mRNA二级结构，促进43S PIC在m 7 G帽处或靠近m 7 G帽处的招募，形成mRNA 43S复合体。 eIF1和1A分别与40S亚单位的P和A位点结合，在40S的头部和主体之间打开一个裂缝，有利于单链mRNA的结合和随后的扫描步骤。 43S PIC中的eIF3和激活 …

eIF4G binds to the 40S subunit through multiple contacts. Human eIF4G uses multiple interactions to form a molecular bridge that connects the eIF4F cap-binding complex and the 40S subunit. An eIF3-specific binding domain (colored green) in human eIF4G indirectly stabilizes eIF4G on the 40S subunit.

eIF3可以与40 S小亚基的多个位点结合，环绕整个小亚基，还可与多种起始因子相互作用，募集并调节其精细定位，从而形成多因子复合物（MFC）。 酵母和兔的40 S亚基与eIF3复合物。Philos Trans R Soc Lond B Biol Sci. 2017 Mar 19; 372(1716): 20160186. eIF3具有多种功能。

2024年4月4日 · Messenger RNA (mRNA) recruitment to the 40S ribosomal subunit is mediated by eukaryotic initiation factor 4F (eIF4F). This complex includes 3 subunits: eIF4E (m 7 G cap binding protein), eIF4A (DEAD-box helicase), and eIF4G. Mammalian eIF4G is a scaffold that coordinates the activities of e …

Messenger RNA (mRNA) recruitment to the 40S ribosomal subunit is mediated by eukaryotic initiation factor 4F (eIF4F). This complex includes three subunits: eIF4E (m7G cap-binding protein), eIF4A (DEAD-box helicase), and eIF4G.

2023年9月30日 · Mammalian eIF4G is a scaffold that coordinates the activities of eIF4E and eIF4A and provides a bridge to connect the mRNA and 40S ribosomal subunit through its interaction with eIF3. While the roles of many eIF4G binding domains are relatively clear, the precise function of RNA binding by eIF4G remains to be elucidated.

2023年9月29日 · Messenger RNA (mRNA) recruitment to the 40S ribosomal subunit is mediated by eukaryotic initiation factor 4F (eIF4F). This complex includes 3 subunits: eIF4E (m7G cap binding protein), eIF4A...

哺乳动物 eIF4G 是协调 eIF4E 和 eIF4A 活性的支架，并通过与 eIF3 的相互作用提供连接 mRNA 和 40S 核糖体亚基的桥梁。 虽然许多 eIF4G 结合域的作用相对清楚，但 eIF4G 结合 RNA 的精确功能仍有待阐明。

2024年1月29日 · Eukaryotic translation initiation involves recruitment of the 43S pre-initiation complex to the 5′ end of mRNA by the cap-binding complex eIF4F, forming the 48S translation initiation complex...