2012年5月1日 · Recently a homogeneous time resolved fluorescence assay for IP1 (IP-One HTRF ®), has been developed by Cisbio (see below). This assay format takes advantage of …

免疫沉淀技术（Immunoprecipitation, IP）：一种利用特异性抗体结合抗原，并通过沉淀富集的方式从复杂的样本中分离出目标蛋白的方法，可用于检测目标蛋白与其他蛋白或DNA片段之间的 …

HTRF IP-one系列产品是针对Gq蛋白GPCR的分析平台。 该产品通过抑制通路中IP-One的降解，来实现稳定检测Ca流通路的目的，可检测缓慢结合的GPCR。 (如图示)

HTRF IP-One assays detect the accumulation of inositol monophosphate, a stable downstream metabolite of IP3 induced by activation of a phospholipase C (PLC) cascade. This kit allows …

This IP-One assay measures the accumulation of IP1 instead of the transient second messengers IP3 and IP2 and is proven effective as a surrogate measure of GPCR activation. The detection …

a) Cisbio IP1 assays实验设计原理：IP-1 assays针对的是与Gq蛋白结合的GPCR，该方法测定的对象是细胞内IP1的浓度。 当Gq蛋白与GPCR结合后，这会引发细胞内瞬时大量IP3和瞬时的 …

HTRF IP1测量试剂盒是基于HTRF技术的竞争性免疫检测，使用了铽穴状化合物（Tb cryptate）标记的抗IP1单抗和d2标记的IP1。 细胞所产生的IP1和试剂盒所提供的标记了d2的IP1竞争抗IP1 …

2012年5月1日 · This chapter describes technologies that can be used to develop robust assays for screening compounds, more specifically the use of Homogeneous Time-Resolved …

Revvity offers an innovative approach with IP-One, a non-radioactive, homogeneous, inositol-phosphate accumulation assay for Gαq signaling. This kit has been shown to offer superior …

Revvity HTRF® IP-One assay is a highly reproducible, reliable technique for downstream measurement of Gαq-coupled GPCR receptor activation. This technical note presents data …