
工具鼠的工具鼠,Reporter 鼠简介 - 知乎 - 知乎专栏
常见的有 TdTomato鼠 、 mT/mG鼠 、 LacZ鼠 、彩虹鼠等,他们的重要作用是对各种特异性表达的Cre工具鼠进行验证,简而言之就是“工具鼠的工具鼠”:前面我们关于 Cre-loxp系统 的介绍 …
肠道谱系追踪方法 - 知乎 - 知乎专栏
2023年9月21日 · 结合Cre-loxp系统,可以实现对基因表达的时间和细胞类型的特异性控制。 更重要的是,作为一种靶向基因敲入策略,所需的小鼠数量比BAC转基因和PiggyBac转基因要 …
Cre报告基因品系 (Cre Reporter) - 哔哩哔哩
2024年6月13日 · 当Cre重组酶切割loxP位点时,lacZ基因被激活,β-半乳糖苷酶的表达可以通过X-gal染色来检测。 用途:用于检测Cre重组酶的活性,通过蓝色染色标记表达lacZ的细胞。
A novel reporter rat strain that expresses LacZ upon Cre-mediated ...
The LacZ541 rat enables evaluation of the performance of Cre-expressing systems which are based upon transgenic rats or somatic gene transfer vectors and provides efficient and simple …
Mef2c-F10N enhancer driven β-galactosidase (LacZ) and Cre …
2015年6月1日 · We describe Mef2c-F10N -LacZ and Mef2c-F10N -Cre stable transgenic mouse lines. These lines mice facilitate tracing migratory NCC. These lines allow for analyzing gene …
Generalized lacZ expression with the ROSA26 Cre reporter strain
Mouse strains expressing the site-specific recombinase Cre (or Flp) facilitate conditional ablation of gene function when one or several exons of the gene of interest are flanked by loxP (or FRT)...
在Cre介导的重组后表达LacZ的新型报告基因大鼠品系。,genesis
2013年2月25日 · 我们已经产生了条件性(Cre / loxP依赖性)LacZ报告基因大鼠(称为LacZ541大鼠)来监测转基因大鼠中的Cre。 当在CAG启动子的控制下,将LacZ541大鼠与另一种表 …
Cre reporter strains produced by targeted insertion of
Several Cre reporter strains of mice have been described, in which a lacZ gene is turned on in cells expressing Cre recombinase, as well as their daughter cells, following Cre-mediated …
A novel reporter rat strain that expresses LacZ upon Cre‐mediated ...
2013年1月24日 · These models benefit from the ability to monitor the expression and functionality of Cre protein. We have generated a conditional (Cre/ loxP dependent) LacZ reporter rat …
Specific expression of lacZ and cre recombinase in fetal thymic ...
2007年6月18日 · Our analysis shows that the knockin alleles drive expression of Cre or lacZ in all TECs in the fetal thymus. Furthermore, the knockin alleles express Cre or lacZ in a Foxn1-like …