2004年3月7日 · Lefty2 expression was also examined with a Lefty2–lacZ transgene (p, q) in which LacZ expression is driven by the upstream 5.5-kb region of the Lefty2 gene 17.

The lefty2 mutant phenotype was partially suppressed by heterozygosity for nodal, suggesting antagonistic effects of the two proteins. In addition, we found that Lefty2 and Antivin act as antagonists of Nodal signaling in zebrafish and induce phenotypes identical to sqt;cyc and maternal-zygotic oep mutants.

2017年11月14日 · Deletion of Lefty1 alone or together with Lefty2 increased the number of prospective DVE cells. Ablation of L1epi or L1dve cells triggered Lefty1 expression in a subset of remaining cells.

2003年4月1日 · The lack of Nodal expression in left LPM of Nodalneo/neo embryos was further confirmed with the use of a Lefty2 ASE-LacZ transgene that is a sensitive indicator of Nodal activity. The expression of LacZ in this transgene is controlled by the Lefty2 asymmetric enhancer (ASE), which is responsive to Nodal signaling.

2000年12月15日 · The role of Lefty2 in left–right patterning was investigated by analysis of mutant mice that lack asymmetric expression of lefty2. These animals exhibited various situs defects including left isomerism. The asymmetric expression of nodal was prolonged and the expression of Pitx2 was upregulated in the mutant embryos.

2001年1月1日 · Pitx2 is left–right (L–R) asymmetrically expressed initially in the lateral plate and later in primordial visceral organs. The transcriptional regulatory mechanisms that underlie L–R asymmetric expression of Pitx2 were investigated.

2017年2月9日 · We propose a model to explain how left isomerism arises in a proportion of Ccdc40lnks mutants. We postulate that with defective motile cilia, Cerl2 expression remains symmetric and Nodal is antagonized equally on both sides of the node.

2002年4月19日 · The phenotype of lefty2 mutant mice and the genetic interaction between lefty2 and nodal suggest that Lefty antagonizes Nodal activity. Our biochemical data now establish that Lefty indeed inhibits Nodal activity.

2003年5月1日 · Our results indicate that the leftsided expression of Nodal in LPM is initiated by Nodal produced in the node, and that Lefty1 expression at the midline is induced by Nodal produced in left LPM. We propose that Nodal activity travels from the node to left LPM, and from left LPM to the midline.

2004年2月17日 · We report that the TGFβ signaling antagonist Lefty also acts through an EGF-CFC-dependent mechanism. Lefty inhibits Nodal and Vg1 signaling, but not Activin signaling. Lefty genetically interacts with EGF-CFC proteins and …