Stevens等人构建的融合基因含有可诱导的小鼠MT-1 promoter和LacZ基因，用锌诱导处理后，表明MT-1 promoter从小鼠胚胎发育开始就有功能，它在早至1细胞时期即能通过诱导使其后的LacZ基因表达。

Expression patterns of several dozen C. elegans genes have now been investigated by fusing the gene sequences to a lacZ reporter construct, introducing the gene fusion into worms, and then staining for β-galactosidase activity.

2020年7月1日 · Although lacZ dsRNA should not target the C. elegans transcriptome, we wanted to confirm that lacZ (RNAi) was not affecting pop-1 levels. To do this, we performed reverse-transcriptase PCR on worms subjected to RNAi of lacZ, pop-1, or empty pL4440 vector.

2006年4月5日 · The E. coli gene LacZ, encoding β-galactosidase, was first used for single-cell gene expression analysis in C. elegans in 1990 (Harrison et al., 1990) and was the reporter gene of choice until the introduction of GFP in 1994 (Chalfie et al., 1994).

1993年9月15日 · In the vector pJS303, expression of the Escherichia coli lacZ gene is regulated by C. elegans act-4 sequences (Fig. 1). When worms are transformed with this act-4::lac'Z vector and exposed to the chromogenic substrate XGal, they turn blue. Non- …

2023年12月15日 · The lacZ gene of Escherichia coli encodes β-galactosidase (β-gal), a lactose metabolism enzyme of the lactose operon. Previous chemical modification or site-directed mutagenesis experiments have identified 21 amino acids that are …

Strand misalignments at DNA repeats during replication are implicated in mutational hotspots. To study these events, we have generated strains carrying mutations in the Escherichia coli chromosomal lacZ gene that revert via deletion of a short ...

We have analyzed the expression and function of Cecpz-1, a Caenorhabditis elegans cathepsin Z-like cysteine protease gene, during development of the worm. The cpz-1 gene is expressed in various...

We have obtained a line of worms that carry an unstable (presumed extrachromosomal) array of a plasmid containing the act-4 5' and 3' regions fused to the E. coli lacZ coding region.

2023年11月7日 · The lacZ gene of Escherichia coli encodes β-galactosidase (β-gal), a lactose metabolism enzyme of the lactose operon. Previous chemical modification or site-directed mutagenesis experiments have identified 21 amino acids that are …