We report here a method for quantitative measurement of iNOS mRNA levels with rtPCR directly from cells lysed with a single step phenol/chloroform/ether extraction. Using a mouse macrophage cell line, J774.2, which expresses iNOS mRNA upon LPS + IFN-gamma treatment as the model, the effects of the extraction on iNOS mRNA recovery and cytosolic ...

Many potent iNOS inhibitors with high selectivity over related NOS isoforms, neuronal NOS (nNOS) and endothelial NOS (eNOS), have been discovered, and these drugs have shown promise in animal models of endotoxemia, inflammatory and …

2019年7月1日 · siRNA-mediated down-regulation of Upf1 enhances cytokine-induced iNOS- mRNA- and protein-expression. DLD-1 cells were transiently transfected with a specific anti-Upf1 siRNA (siUpf1) and a control siRNA (siCo). After 48 h the cells were preincubated for 18 h in medium without FCS and phenol red.

内毒素、细胞因子、IFNG/ IFN-γ能与细菌脂多糖 (LPS)、TNF或IL1B/白细胞介素-1 β协同作用诱导iNOS的表达。 在肝脏、视网膜、骨细胞和肺的气道上皮细胞中表达。 在血小板中不表达。 细胞质. 图二：iNOS的ICC实验结果图，Anti-iNOS [RM1017]产品 (ab283655)。 绿色：iNOS，红色：alpha Tubulin，蓝色：DAPI。 人（P35228）：异构体1、2：126-131 kDa（预测） 小鼠（P29477）：131 kDa（预测） 大鼠（Q06518）：131 kDa（预测） 多聚泛素化和磷酸化. …

地塞米松和转化生长因子-β均减弱了il-1β和infγ诱导的inos mrna丰度和酶活性。 地塞米松的预处理也消除了iNOS mRNA的诱导，但没有消除注射了脂多糖的大鼠的纯化心肌细胞中GTP环水解酶mRNA的增加。

在培养的牛主动脉内皮细胞，TNFa可下调 eNOS mrNA、酶蛋白及其活性，但不引起iNOS平行诱导，提示下调eNOS表达和诱导iNOS表达可独立出现。 TNF-a可降低 eNOS mRNA稳定性，但对转录可能没有影响。

2010年9月15日 · Increased gastric iNOS mRNA expression was found in subjects with long CCTTT-repeats (either allele >11) in the iNOS promoter analyzing H. pylori-infected patients with gastric cancer, peptic ulcer or functional dyspepsia [56].

Synthesis and localization of inducible nitric oxide synthase mRNA (iNOS-mRNA) and iNOS protein in the cultures of human monocytes (Mphi) and colon carcinoma cell line (DeTa) that resulted in nitric oxide (NO) synthesis has been studied. The iNOS-mRNA was observed around the sixth hour of culture an …

1995年8月1日 · By reverse transcriptase-polymerase chain reaction (RT-PCR) analysis, we show that human monocytes can be induced to express iNOS mRNA after treatment with lipopolysaccharide (LPS) and/or interferon- γ (IFN-γ).

1995年8月1日 · The purpose of this study was to determine if stimulated human mononuclear phagocytes produce inducible nitric oxide synthase (iNOS) mRNA, protein, and enzymatic activity. By reverse transcriptase-polymerase chain reaction (RT-PCR) analysis, we show that human monocytes can be induced to express iNOS mRNA after treatment with lipopolysaccharide ...