2022年8月17日 · Next, we investigated whether mTORC1 regulated lysosomal proteolysis using DQ BSA, a self-quenched albumin probe that fluoresces upon degradation 17. To monitor lysosomal catabolism directly,...

BSA+Gln可通过巨胞饮-内吞进入胞内，和溶酶体融合后被降解成基本氨基酸，转运出溶酶体后再激活mTORC1。 敲低HOPS复合物的表达，干扰内吞溶酶体融合，将阻断BSA+Gln激活mTORC1。

Mammalian/mechanistic target of rapamycin (mTOR) is an evolutionarily conserved genuine protein kinase, which phosphorylates serine/threonine in response to growth factors and nutrients. It functions as a catalytic core in two distinct multiprotein ...

In murine pancreatic cancer cells grown in physiological nutrient conditions, we found that almost half of both intracellular and extracellular amino acid pools were derived from BSA (i.e. unlabeled), demonstrating that protein catabolism can be a major contributor to amino acid pools in pancreatic cancer (Kamphorst et al., 2015).

2017年6月28日 · Our data show that the Akt/mTOR/p70S6K1 pathway induced by PA-BSA coupling with GPR40 directly stimulates APP and BACE1 expressions.

mTORC2 可以通过激活 Akt 促进细胞存活，通过激活 PKCα 调节细胞骨架动力学，以及通过磷酸化 SGK1 控制离子转运和生长。mTOR 信号转导异常见于多种疾病，如癌症、心血管疾病和糖尿病。

2023年9月4日 · 研究表明mTOR在细胞增殖、存活、代谢、自噬、凋亡、迁移等生物学过程中均发挥重要作用，且mTOR介导的信号通路(如PI3K/Akt/mTOR信号通路、mTOR/p70S6K信号通路、AMPK/mTOR信号通路等)参与了诸多人类疾病的发生与发展，并能对机体内多种生物学功能进行调 …

哺乳动物雷帕霉素靶蛋白(mammalian target of rapamycin, mTOR)是一种非典型丝氨酸/苏氨酸蛋白激酶，为磷脂酰肌醇激酶相关激酶(phosphatidy-linositol kinase-related kinase, PIKK)蛋白质家族成员。

Simple Western™ analysis of lysates (1 mg/ml) from MCF-7 cells using mTOR (L27D4) Mouse mAb #4517. The virtual lane view (left) shows the target band (as indicated) at 1:50 and 1:250 dilutions of primary antibody.

2020年10月16日 · The mechanistic target of rapamycin complex 1 (mTORC1) couples nutrient sufficiency to cell growth. mTORC1 is activated by exogenously acquired amino acids sensed through the GATOR–Rag guanosine triphosphatase (GTPase) pathway, or by amino acids derived through lysosomal degradation of protein by a poorly defined mechanism.