The nif genes are genes encoding enzymes involved in the fixation of atmospheric nitrogen into a form of nitrogen available to living organisms. The primary enzyme encoded by the nif genes is the nitrogenase complex which is in charge of converting atmospheric nitrogen (N 2 ) to other nitrogen forms such as ammonia which the organism can use ...

The nif genes can be classified into three subgroups: (1) genes that code for proteins that act as molecular scaffolds for the inorganic clusters. This group includes NifU, NifB, NifQ and NifEN. (2) Genes that function as chaperones that bind, protect, and transport the metal clusters from the point of biosynthesis to the site of incorporation ...

2024年11月23日 · The nif genes are genes encoding enzymes involved in the fixation of atmospheric nitrogen. The primary enzyme encoded by the nif genes is the nitrogenase complex which is in charge of converting atmospheric nitrogen to other nitrogen forms such as ammonia, which plants can use for various purposes.

2018年7月19日 · Transfer of nitrogen fixation (nif) genes from diazotrophs to amenable heterologous hosts is of increasing interest to genetically engineer nitrogen fixation....

We first identify the six genes involved in nitrogen fixation in all sequenced prokaryotic genomes and then reconstruct phylogenetic trees using the six Nif proteins individually or in combination.

2012年5月3日 · Based on a careful comparison of the repertoire of nitrogen fixation genes in known diazotroph species we propose a new criterion for computational prediction of nitrogen fixation: the presence of a minimum set of six genes coding for structural and biosynthetic components, namely NifHDK and NifENB.

nif genes • The two main classes of nitrogen-fixing genes, the nif genes and fix genes, are present in rhizobia. • The nif genes encode nitrogenase and show structural and functional resemblance with the nitrogen-fixing genes present in Klebsiella pneumoniae and other microbial groups. • Although most of the nif genes are found on ...

2014年2月3日 · Metagenomic analyses revealed that 742 sequences were identified as nif genes including structural subunit genes nifH, nifD, nifK and various additional genes. The AMD community is massively dominated by the genus Acidithiobacillus.

2008年3月6日 · We analyzed the levels of nif transcripts of Synechococcus ecotypes, NifH subunit and nitrogenase activity over the diel cycle in the microbial mat of an alkaline hot spring in Yellowstone...

2025年1月27日 · This study advances our understanding of the distribution of nif genes, aiding in the prediction and classification of N₂-fixing organisms. Furthermore, we present a comprehensive overview of the diversity, distribution, and evolutionary relationships among diazotrophic organisms associated with the …