pACYC184 Sequence and Map - SnapGene
Plasmid containing the p15A origin of replication and a tetracycline resistance gene. Sequence Author: New England Biolabs. Explore Over 2.7k Plasmids: Basic Cloning Vectors | More Plasmid Sets. Sticky ends from different BsrDI sites may not be compatible. Sticky ends from different BaeGI sites may not be compatible.
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pACYC184 - NEB
pACYC184 pACYC184 is an E. coli plasmid cloning vector containing the p15A origin of replication (1-4). This allows pACYC184 to coexist in cells with plasmids of the ColE1 compatibility group (e.g., pBR322, pUC19). It is a low copy number vector, at about 15 copies per cell (5), but can be amplified with spectinomycin.
pACYC184质粒图谱、载体图谱、序列、价格、抗性、测序引物、 …
pACYC184载体价格800元，pACYC184载体质粒图谱(Vector map),载体序列(Sequence)见下文，载体质粒抗性为氯霉素，质粒大小为4245bp，是大肠杆菌双表达系统的pACYC184载体。
pACYC184_载体及菌株库_产品中心_奥诺基因-HonorGene-基因研 …
pACYC184 is an E. coli plasmid cloning vector containing the p15A origin of replication. This allows pACYC184 to coexist in cells with plasmids of the ColE1 compatibility group (e.g., pBR322, pUC19). It is a low copy number vector, at about 15 copies per cell, but can be …
pACYC184 载体图谱和序列 - 纽普生物
提供 pacyc184 载体/质粒图谱，全长序列，抗性，大小和元件信息。
pACYC184-Trc-mKATE2-Trc-MCS 载体图谱和序列 - 纽普生物
提供 pACYC184-Trc-mKATE2-Trc-MCS 载体/质粒图谱，全长序列，抗性，大小和元件信息。
Addgene: Vector Database - pACYC184
There is a problem with the plasmid I received. What strain of bacteria does my stab contain? How can I be notified when a new material becomes available? What is virus associated DNA, and why do I have to order it? Welcome to Vector Database!
pACYC184,pACYC184 生命科学产品与技术服务-生工生物工程 (上 …
本产品/服务仅供科研使用. 1.溶解： 收到质粒干粉后，请加入 20μl 无菌水至管底，溶解质粒，室温静置 1min； 2.混合（吸附质粒）： 200μl 感受态细胞 + 质粒 DNA 5~10µl 混匀，冰上放置 30min； 3.热激导入： 42℃静置 90s； 4.收缩膜孔： 冰浴 2min； 5.修复培养： 毎管加 800µl LB 液体培养基，37 ℃培养 1h 150 r/min； 6.筛选培养： 将适当体积（100 µl）的复苏细胞，涂布在相应抗性的 LB 平板上，正置平皿 30min（琼脂面务必干燥后）， 倒置培养 12-16h，出现菌落 …
pACYC184质粒-北京庄盟国际生物基因科技有限公司
pACYC184 is an E. coli plasmid cloning vector containing the p15A origin of replication (1-4). This allows pACYC184 to coexist in cells with plasmids of the ColE1 compatibility group (e.g., pBR322, pUC19). It is a low copy number vector, at about 15 copies per cell (5), but can be …
pACYC184大肠杆菌表达载体说明 - 豆丁网
2016年12月11日 · 北京华越洋VECT-140pACYC184. pACYC184载体基本信息. 载体名称:pACYC184. 质粒类型:原核表达系统载体. 高拷贝/低拷贝:低拷贝. 启动子:-- 克隆方法:多克隆位点，限制性内切酶. 载体大小:4245bp. 5'测序引物及序列:-- 3'测序引物及序列:-- 载体标签:-- 载体抗性:Chloramphenicol. 筛选标记:Tetracycline. 备注:-- 稳定性:-- 组成型:-- 病毒/非病毒:-- pACYC184载体质粒图谱和多克隆位点信息. pACYC184载体简介. …