pBR322 is a plasmid and was one of the first widely used E. coli cloning vectors. Created in 1977 in the laboratory of Herbert Boyer at the University of California, San Francisco, it was named after Francisco Bolivar Zapata, the postdoctoral researcher and Raymond L. Rodriguez. The p stands for "plasmid," and BR for "Bolivar" and "Rodriguez."

First-generation E. coli vector for DNA cloning. This plasmid has a low copy number (~20 copies per cell) due to the rop gene.

2023年8月3日 · pBR322 is a commonly used cloning vector in E. coli and has tremendous applications in cloning. It was constructed in 1977 in the lab of Herbert Boyer at The University of California in San Francisco. It is a synthetic plasmid and was the first artificial plasmid to be constructed and used as a cloning vector.

pBR322 DNA is a commonly used plasmid cloning vector in E. coli (1). The molecule is a double-stranded circle 4,361* base pairs in length (2). pBR322 contains the genes for resistance to ampicillin and tetracycline, and can be amplified with chloramphenicol.

2023年5月12日 · The pBR322 vector is an essential tool in molecular biology and genetic engineering due to its unique combination of antibiotic resistance genes, restriction enzyme sites, and other important features.

pBR322 is an E. coli plasmid cloning vector containing the origin of replication from pMB1 (a plasmid in the ColE1 com-patibility group; 1–3). The rop gene product, which regulates plasmid replication by stabilizing the interaction between RNAI and RNAII transcripts, maintains the copy number at about 20 per cell.

2024年7月5日 · At its core, pBR322 is a circular double-stranded DNA molecule, approximately 4,361 base pairs in length. This compact size makes it an ideal vector for cloning, as it can be easily manipulated and introduced into host cells.