Vector for fusing EGFP to the N-terminus of a partner protein. For other reading frames, use pEGFP-C2 or pEGFP-C3.

pEGFP-C1 encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized for brighter fluorescence and higher expression in mammalian cells. (Excitation maximum = 488 nm;emission maximum = 507 nm.) pEGFP-C1 encodes the GFPmut1 variant (4) which contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr.

Plasmid: pEGFP-C1. Information Source/Vendor Clontech Alt Name EGFP C1, pEGFPC1, EGFPC1 Plasmid Type Mammalian Expression Promoter CMV Expression Level High Cloning Method Restriction Enzyme Size 4731 5' Sequencing 1 Primer EGFP-C 5' Sequencing 1 Primer Sequence CATGGTCCTGCTGGAGTTCGTG 3' Sequencing 1 Primer

pEGFP-C1 encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized for brighter fluorescence and higher expression in mammalian cells. (Excitation maximum = 488 nm; emission maximum = 507 nm.) pEGFP-C1 encodes the GFPmut1 variant (4) which contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr.

The pEGFP-C1 vector features an enhanced green fluorescent protein (EGFP) gene. Its multiple cloning site is located after the GFP gene, enabling the N-terminus of the target protein to be fused with GFP in the constructed fusion protein. Commonly used in molecular biology research, it offers a useful tool for observing protein localization and ...

pEGFP-C1 encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized for brighter fluorescence and higher expression in mammalian cells. (Excitation maximum = 488 nm; emission maximum = 507 nm.) pEGFP-C1 encodes the GFPmut1 variant (4) which contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr.

Plasmid pEGFP-C1-FLAG from Dr. Steve Jackson's lab contains the insert FLAG tag and is published in J Cell Biol. 2013 Jul 29. This plasmid is available through Addgene. More than 20 requests

pEGFP-C1编码野生型GFP（1-3）的红移变体，该变体已经过优化，可以在哺乳动物细胞中获得更亮的荧光和更高的表达。（最大激发波长= 488 nm；最大发射波长= 507 nm。）pEGFP-C1编码GFPmut1变体（4），其中含有Phe-64取代为Leu和Ser-65取代为Thr的双氨基酸。

The pEGFP-C1 vector features an enhanced green fluorescent protein (EGFP) gene. Its multiple cloning site is located after the GFP gene, enabling the N-terminus of the target protein to be fused with GFP in the constructed fusion protein. Commonly used in molecular biology research, it offers a useful tool for observing protein localization and ...

提供 pEGFP-C1 F-tractin-EGFP 载体/质粒图谱，全长序列，抗性，大小和元件信息。