Bacterial vector for expression of N-terminally 6xHis-tagged proteins with a thrombin site. For other reading frames, use pET-28b (+) or pET-28c (+). Sequence Author: MilliporeSigma …

There is a problem with the plasmid I received. What strain of bacteria does my stab contain? How can I be notified when a new material becomes available? What is virus associated DNA, and why do I have to order it? Welcome to Vector Database!

pET-28a (+) is a bacterial expression vector designed for the production of N-terminally 6xHis-tagged proteins with a thrombin cleavage site. This vector is particularly suitable for proteins that require post-translational processing to remove the His-tag. 1. Centrifuge at 5,000×g for 5 min. 2.

pET-28a-c (+)载体带有一个N端的His/Thrombin/T7蛋白标签，同时含有一个可以选择的C端His标签。 pET28a载体的单一的多克隆位点见上面的环状质粒图谱。 注意：载体序列是以pBR322质粒的编码规矩进行编码的，所以T7蛋白表达区在质粒图谱上面是反向的。 T7 RNA聚合酶启动的克隆和表达区域在质粒图谱中也被标注了出来。 质粒的F1复制子是被定向的，所以在T7噬菌体聚 …

Plasmid pET-28a (+)::NL from Dr. Andrew Millar's lab contains the insert NLUC and is published in Plant Methods. 2019 Jul 8;15:68. doi: 10.1186/s13007-019-0454-4. eCollection 2019. This plasmid is available through Addgene.

pET-28a(+) DNA - Novagen Novagen′s pET-28a-c(+) vectors carry an N-terminal His•Tag/thrombin/T7•Tag configuration plus an optional C-terminal His•Tag sequence.; Synonyms: cloning vector,expression vector,pET Plasmid,vector at Sigma-Aldrich

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker. Midwest Center for Structural Genomics.

pET-28a (+) is a bacterial expression vector designed for the production of N-terminally 6xHis-tagged proteins with a thrombin cleavage site. This vector is particularly suitable for proteins that require post-translational processing to remove the His-tag. 1. 发货形式：质粒干粉 (常温运输，存于-20度，请务必先转化提质粒后使用) 2.

All of the pET vectors and companion products are available as kits designed for convenient cloning, expression, detection, and purification of target proteins. The pET-28a-c(+) vectors carry an N-terminal His•Tag®/thrombin/T7•Tag® configuration …