Created by Moore, July 1995, under contract with NCBI. MCS, oligonucleotide linker. Hosts: E.coli. Related vectors: pGEX-2T. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).) How was your experience today? Addgene is a nonprofit plasmid repository.

Efficient cleavage requires at least two copies of the NarI recognition sequence. EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage. BssHII is typically used at 50°C, but is 75% active at 37°C. ApaI can be used between 25°C and 37°C. Sticky ends from different BanII sites may not be compatible.

pGEX-3X载体价格800元，pGEX 3X载体质粒图谱(Vector map),pGEX3X载体列(Sequence)和多克隆位点见下文，载体抗性为氨苄，质粒大小为4952 bp，测序引物为pGEX5'和pGEX3'，主要用于大肠杆菌蛋白表达.

pGEX-1_lambda_T. pGEX-4T-1. pGEX-5X-1. pGEX-6P-1. pGEX-2T. pGEX-4T-2. pGEX-5X-2. pGEX-6P-2. pGEX-2TK. pGEX-4T-3. pGEX-5X-3. pGEX-6P-3. pGEX-3X. Plasmid Sets. Basic Cloning Vectors. I.M.A.G.E. Consortium Plasmids. pET & Duet Vectors (Novagen) TA and GC Cloning Vectors. Coronavirus Resources. Insect Cell Vectors.

最近看了一些文献，大家一致公认原核表达载体以PGEX系列较好，但PGEX系列有PGEX-3X 、 PGEX-4T-1 、 6P-1、4T-2等多种不同载体，具体在实验中应该怎么样来选择载体呢？ 在选择时有个什么准则没有？ 主要区别就是:1 多克隆位点 (MCS)酶切点不同,如何选择上边的说得很清楚呢; 2 GST和MCS之间均含有凝血酶或者Xa因子的切割位点.看你以后想用什么蛋白酶来切割目的蛋白.公司的这两种酶都点贵,至少600吧.凝血酶我们用医院买的就可以,只要几十块,还不知道医院有没 …

提供 pgex-puc-3t 载体/质粒图谱，全长序列，抗性，大小和元件信息。

来自GE Healthcare的谷胱甘肽转移酶(GST) 基因融合系统是一种表达、纯化和检测大肠杆菌中所产生的GST标签蛋白的多功能系统。系统包括三个主要成分: pGEX质粒表达载体、GST纯化的产品以及一系列GST检测产品。一系列切割GST标签的位点特异蛋白酶则是该系统的补充。

Collectively, the pGEX vectors provide all three translational reading frames beginning with the EcoRI restriction site (Figure 1.1). pGEX-1λT, pGEX-6P-1, pGEX-4T-1, and pGEX-5X-1 can directly accept and express cDNA inserts isolated from λgt11 libraries.

提供 pgex-3x 载体/质粒图谱，全长序列，抗性，大小和元件信息。

Provide pGEX-PUC-3T vector/plasmid map, full length sequence, antibiotic resistance, size and other information