Explore Bio-Rad’s comprehensive pGLO and GFP teaching resources, including pGLO plasmid maps, hands-on lab activities, and free presentations, videos, and case studies. Learn how to use GFP to illuminate gene regulation and expression in your classroom.

Bio-Rad’s unique pGLO plasmid contains the gene for GFP and a gene for resistance to the antibiotic ampicillin. pGLO also incorporates a special gene regulation system that can be used to control expression of the fluorescent protein in transformed cells.

Bio-Rad’s pGLO plasmid has three special genes: one for GFP, a gene for antibiotic resistance, and a gene regulation system. This system can be used to control when the bacteria produce fluorescent protein. The gene for GFP can be switched on in transformed cells by adding the sugar arabinose to the cells’ food source.

pGLO载体价格1500元，pGLO载体质粒图谱(Vector map)，载体序列(Sequence)见下文，载体质粒抗性为氨苄青霉素，质粒大小为5371 bp。

• Table 1 shows plates with E. coli cells that contain the pGLO plasmid and demonstrate bacterial growth • A limitation of this study would be that we expressed the GFP gene of the pGLO

Each table can be divided into 3 groups; one group will work on A- mutant one on G- mutant and the third on pGLO controls. The whole table will then make a gel and load their samples.

In this activity, you will have the opportunity to genetically transform bacteria cells; altering them so that they. can make an entirely new protein. This procedure is used widely in biotechnology laboratories all over the world, enabling scientists to manipulate and study genes and proteins in exciting new ways.

bacteria and pGLO, a plasmid modified with three genes. The pGLO plasmid contains the genetic codes for (see Table 2): 1. a green fluorescent protein (GFP) from the bioluminescent jellyfish, Aequorea victoria 2. ampicillin resistance (amp. R) 3. a special gene regulation system (araC) requiring the sugar arabinose in the media to induce GFP ...

Part 1. Transform Bacteria with the pGLO Plasmid A. In Table 1 below, sketch and describe the results you expect after you complete the experiment. Table 1. Predicted results. LB Plate (LB) LB Plate with ampicillin (LB/amp) Description

Reasons for Performing Each Transformation Step? Why Perform Each Transformation Step? 2. Incubate on ice. 3. Heat-shock. 4. Nutrient broth incubation. What is Nutrient Broth? Grow? Glow? On which plates will colonies grow? Which colonies will glow?