Explore Bio-Rad’s comprehensive pGLO and GFP teaching resources, including pGLO plasmid maps, hands-on lab activities, and free presentations, videos, and case studies. Learn how to …

The pGLO plasmid contains an origin or replication, a selectable marker, and the gene for Green Fluorescent Protein (GFP). The plasmid also contains a gene for the arabinose C protein, …

The pGLO plasmid is an engineered plasmid used in biotechnology as a vector for creating genetically modified organisms. The plasmid contains several reporter genes, most notably the …

2019年1月1日 · The Bio-Rad pGLO bacterial transformation kit is commonly used to demonstrate this form of genetic exchange, which occurs in bacteria and eukaryotes and which differs …

2011年11月11日 · In this project, the EZ-Tn5 transposon is used to generate and screen a large number of cells transformed with mutagenized pGLO plasmid. EZ-Tn5 carries the kanamycin …

In this bacterial transformation lab activity, students use the pGLO plasmid to transform bacteria to express green fluorescent protein (GFP) from the bioluminescent jellyfish Aequorea victoria, …

The pGLO plasmid contains the gene for GFP and a gene for resistance to the antibiotic ampicillin. pGLO also incorporates a special gene regulation system that can be used to …

pGLO is a trademarked engineered plasmid widely used in genetic research to create genetically modified organisms (GMOs). This plasmid has been specifically designed to include several …

Why Perform Each Transformation Step? 2. Incubate on ice. 3. Heat-shock. 4. Nutrient broth incubation. What is Nutrient Broth? Grow? Glow? On which plates will colonies grow? Which …

2023年11月17日 · The pGLO plasmid contains a gene (bla) for a protein called β-lactamase, which hydrolyzes antibiotics that have a β-lactam ring and makes host cells resistant to …