Perilipin 5 (PLIN5) is a lipid-droplet-associated protein that coordinates intracellular lipolysis in highly oxidative tissues and is thought to regulate lipid metabolism in response to phosphorylation by protein kinase A (PKA). We sought to identify PKA …

2021年1月1日 · We sought to identify PKA phosphorylation sites in PLIN5 and assess their functional relevance in cultured cells and the livers of mice. We detected phosphorylation on S155 and identified S155 as a functionally important site for lipid metabolism.

Perilipin (PLIN) 5 是一种脂滴相关蛋白，可协调高度氧化组织中的细胞内脂肪分解，并被认为可响应蛋白激酶 A (PKA) 的磷酸化来调节脂质代谢。我们试图鉴定 PLIN5 中的 PKA 磷酸化位点，并评估其在培养细胞和小鼠肝脏中的功能相关性。

2015年1月16日 · Here, we show that the lipolytic barrier of Plin5-enriched LDs, either prepared from cardiac tissue of CM-Plin5 mice or Plin5-transfected cells, is abrogated by incubation with PKA. Notably, PKA-induced lipolysis of LDs enriched with Plin5 carrying a single mutation at serine 155 (PlinS155A) of the putative PKA phosphorylation site was ...

2022年3月31日 · However, when the body is under stress (such as cold, fasting, exercise, and other stimuli), protein kinase A (PKA) phosphorylates and activates Plin5, which then causes Plin5 to release the binding site of CGI-58 and ATGL, prompting CGI-58 to bind to ATGL and activate ATGL activity, thus accelerating the lipolysis process, revealing the ...

目的:探讨蛋白激酶A(PKA)信号通路是否参与Perilipin5(Plin5)抑制肝星状细胞(HSC)增殖和诱导其凋亡.方法:将大鼠原代HSC(HSC-Primary)和细胞株HSC-T6感染Plin5慢病毒,Western blot法检测细胞中Plin5、t-PKA、磷酸化PKA(p-PKA)的表达.将两种细胞分别分成4组:空白对照组(不感染)、过表达 ...

The multiple functions of Plin5 may be conferred through its interaction with ATGL and ABHD5 [45–47] and its ability to increase lipolysis through PKA stimulation . Plin5 increases lipolysis in response to PKA activation and can amplify the effects of PKA and cAMP signaling pathways on insulin secretion . After cAMP/PKA-mediated lipid ...

determine PKA-sensitive PLIN5 phosphorylation sites, to assess the metabolic roles of PLIN5 phosphorylation in vitro, and to ascertain whether these effects are

We sought to identify PKA phosphorylation sites in PLIN5 and assess their functional relevance in cultured cells and the livers of mice. We detected phosphorylation on S155 and identified S155 as a functionally important site for lipid metabolism.

2016年12月6日 · PLIN5 facilitates fat storage into LD and inhibits FA oxidation in the resting state while sharply boosting IMTG lipolysis and FA oxidation during muscle contraction or PKA stimulation .