碧云天生产的碱性磷酸酶检测试剂盒 (Alkaline Phosphatase Assay Kit)是一种用于快速、便捷地检测细胞或组织样品的裂解或匀浆产物的上清液、血清、血浆、尿液等样品中内源性的碱性磷酸酶活性的试剂盒。 碱性磷酸酶 (Alkaline Phosphatase, AP/ALP/AKP/ALKP/ALPase/Alk Phos)也称碱性磷酸酯酶 (EC 3.1.3.1)，可以在碱性条件下催化磷酸酯键的水解。 哺乳动物中，肝脏、胆管、肾脏、骨头和胎盘中的碱性磷酸酶活性比较高。

2020年3月18日 · 碱性磷酸酶（Alkaline phospharase, ALP）与骨骼、肝脏等人类疾病的发生有关，是体外诊断试剂中常用的标记酶之一。 ALP测定方法主要是比色法和连续监测法。

2019年4月12日 · The purpose of this manuscript is to develop a quick and reliable assay to measure ALP activity in S. aureus biofilm that does not require protein isolation. Using p-nitrophenyl phosphate (pNPP) as a substrate, we measured ALP activity in S. aureus biofilm formed in 96-well tissue culture plates.

2019年4月1日 · Alkaline phosphatase (ALP) has become a representative enzyme of modern life industries. As a critical biomarker of numerous diseases and a major tool enzyme of experiments, classic methods for ALP assays fail to meet the emerging demand of sensitivity, precision, interference immunity and wide targeted types of sample.

2006年11月3日 · A very robust and inexpensive kinetic assay for determining rates of hydrolysis of p -nitrophenyl phosphate by the enzyme alkaline phosphatase is presented. The reaction increases in rate with increase in pH.

2020年2月13日 · Alkaline phosphatase is also a popular enzyme conjugated with secondary antibody for ELISA. p-Nitrophenyl phosphate (pNPP) is proven to be an effective chromogenic substrate for alkaline phosphate. This SensoLyte® kit can be used to detect alkaline phosphatase activity in biological samples and alkaline phosphatase-based ELISA.

2019年2月15日 · We present a practical electrochemical method for ALP activity in real samples. The strategy has an excellent correlation with the reference colorimetric assay. The method works without interference of human serum and saliva samples. The electrochemical assay quantifies bone ALP isoform in human serum.

In this study we aim to develop more efficient, economical, fast and consistent method to estimate ALP activity and protein concentration in a single tube for cultured cells. Some minor modifications were incorporated in the existing protocol [10] and extensive optimizations were performed.

2016年12月4日 · 对硝基苯酚磷酸盐（pNPP）是一种常用的碱性磷酸酶（ALP）显色底物，碱性条件下能被ALP催化生成对硝基酚（para-nitrophenol，p-nitrophenol），呈黄色产物，可在400-415nm检测到吸光度。

Determination with p-Nitrophenylphosphate (pNPP) A modification of the method by Bergmeyer et al. (1965) is used to determine ALP activity in culture media and cells. PNP is used as the substrate for the determination of ALP activity. After 30-minute incubation the phosphatase is completely inhibited by NaOH and the pNPP liberated form a yellow ...