Standard E. coli vector with a multiple cloning site (MCS) for DNA cloning. The MCS is reversed in pUC19. Explore Over 2.7k Plasmids: Basic Cloning Vectors | More Plasmid Sets. Sticky ends from different EcoO109I sites may not be compatible. Sticky ends from different TsoI sites may not be compatible.

Plasmid pUC18 from Dr. Joachim Messing's lab is published in Gene. 1983 Dec;26(1):101-6. This plasmid is available through Addgene.

pUC18是适合于双脱氧法DNA测序的载体，通常运用于重组dna的分子克隆中，首先我们制备大肠杆菌的感受态细胞（能接受外来重组的dna），这种感受态菌株必须不同外来DNA分子发生遗传重组，通常是rec基因缺陷型的突变体，同时它们必须是限制系统缺陷或限制与 ...

提供 pUC18 载体/质粒图谱，全长序列，抗性，大小和元件信息。

pUC18是一种由pBR322改造而来的质粒载体，全长2686bp，含有lacZ蛋白N端的部分编码序列，在多克隆位点(Multiple cloning site, MCS)插入外源序列后可以通过蓝白斑筛选阳性克隆。

pUC18 is a commonly used plasmid cloning vector in E.coli. The molecule is a double-stranded circular DNA (2686 base pairs in length). Due to a small size pUC18 enables successful cloning of large DNA fragments. The pUC18 plasmid confers ampicillin resistance and complement defects in β-galactosidase in appropriate host strains. The

pUC18是适合于双脱氧法DNA测序的载体，通常运用于重组dna的分子克隆中，首先我们制备大肠杆菌的感受态细胞（能接受外来重组的dna），这种感受态菌株必须不同外来DNA分子发生遗传重组，通常是rec基因缺陷型的突变体，同时它们必须是限制系统缺陷或限制与 ...

Thermo Scientific pUC18 vector is a small, high copy number, E. coli plasmid, 2686 bp in length. It contains identical multiple cloning site (MCS) as pUC19 vector except that it is arranged in opposite orientation.Highlights

2023年3月7日 · pUC18 和 pUC19 大小只有 2686bp ，是最常用的质粒载体，其结构组成紧凑，几乎不含多余的 DNA 片段，GenBank注册号为 L08752(pUC18)和 X02514(pUC19)。 由 pBR322 改造而来，其中 lacZ (MSC)来自 M13mp18/19 图 3-4 是其质粒图谱 。

The pUC18 and pUC19 plasmids enable successful cloning of large DNA fragments (larger than those cloned with a M13 mp18 RF Phage Vector).