Standard E. coli vector with a multiple cloning site (MCS) for DNA cloning. The MCS is reversed in pUC19.

Plasmid pUC18 from Dr. Joachim Messing's lab is published in Gene. 1983 Dec;26 (1):101-6. This plasmid is available through Addgene.

提供 pUC18 载体/质粒图谱，全长序列，抗性，大小和元件信息。

Discrepancies between sequencing results obtained by Addgene and the original sequence provided by the depositor may be present. Addgene has sequenced portions of this plasmid for verification. The results are shown below. Leading primers are indicated on the first line of each sequence. How was your experience today?

pUC18 is a commonly used plasmid cloning vector in E.coli. The molecule is a double-stranded circular DNA (2686 base pairs in length). Due to a small size pUC18 enables successful cloning of large DNA fragments. The pUC18 plasmid confers ampicillin resistance and complement defects in β-galactosidase in appropriate host strains. The

The pUC18 and pUC19 plasmids enable successful cloning of large DNA fragments (larger than those cloned with a M13 mp18 RF Phage Vector).

Thermo Scientific pUC18 vector is a small, high copy number, E. coli plasmid, 2686 bp in length. It contains identical multiple cloning site (MCS) as pUC19 vector except that it is arranged in opposite orientation. Highlights • Purified by chromatography using proprietary patented technology • More than 90% in the supercoiled form

pUC18 high copy cloning vector for replication in E. coli, suitable for “blue-white screening” technique. Description pUC18 is a small, high copy cloning vector for replication in E. coli. It has been constructed using the ampicillin resistance gene and the pMB1 origin of replication from pBR322. The pMB1 of pUC18 differs

pUC18/19 Vector Map Multiple Cloning Sites of pUC18: M13 Hind lll Bev l

pUC18 is a plasmid cloning vector commonly used with E. coli. The vector length is 2686 bp and is isolated from E. coli strain DH5α by standard procedures. Supplied as 50 ug lyophilized powder in TE buffer (10 mM Tris-HCl and 1 mM EDTA, pH 7.5). Store at -20°C.