Plasmid pUC57-sgRNA expression vector from Dr. Xingxu Huang's lab contains the insert sgRNA scaffold and is published in Nat Methods. 2014 Mar 2. doi: 10.1038/nmeth.2857. This plasmid is available through Addgene.

提供 pUC57 载体/质粒图谱，全长序列，抗性，大小和元件信息。

pUC57-sgRNA expression vector Plasmid for in vitro transcription of a single guide RNA (sgRNA) using the T7 promoter. Sequence Author : Huang Lab / Addgene #51132

After cleavage, TsoI can remain bound to DNA and alter its electrophoretic mobility. For full activity, add fresh S-adenosylmethionine (SAM). Efficient cleavage requires at least two copies of the NmeAIII recognition sequence. Sticky ends from different NmeAIII sites may not be compatible. For full activity, add fresh S-adenosylmethionine (SAM).

质粒简介： pUC57-sgRNA质粒是一个用于体外转录sgRNA的载体，gRNA片段可通过BsaI酶切连入，并在T7启动子的驱动下转录出来。本质粒的参考文献是：Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects.

pUC57-sgRNA质粒价格1200元，pUC57-sgRNA (爱迪基因 51132 )质粒图谱（map），质粒序列 (Sequence)见下文，质粒抗性为卡那霉素。

pUC57 Simple 通用克隆载体消除了大部分的常用酶切位点，便于后续亚克隆。 该产 品适用于Taq 酶扩增的含“A”尾巴和高保真酶扩增的PCR 产物的连接，载体含有氨苄青霉素抗性筛选标记。

Derivative of the standard cloning vector pUC57 with only an EcoRV site available for cloning. Explore Over 2.7k Plasmids: Basic Cloning Vectors | More Plasmid Sets. Sticky ends from different EcoO109I sites may not be compatible. Sticky ends from different TsoI sites may …

PUC57-Simple-T7-gRNA 载体 (vector) 是在基因工程重组 DNA 技术中将 DNA 片段（目的基因）转移至受体细胞的一种能自我复制的 DNA 分子。三种最常用的载体是细菌质粒、噬菌体和细菌病毒和动植物病毒。

提供 pUC57-sgRNA 载体/质粒图谱，全长序列，抗性，大小和元件信息。