The piggyBac transposon was originally isolated from the cabbage looper moth, Trichoplusia ni, in the 1980s. Despite its early discovery and dissimilarity to the other DNA transposon families, the piggyBac transposon was not recognized as a member of a large transposon superfamily for a …

2025年2月12日 · Explore the intricate structure and function of PiggyBac transposase, focusing on its DNA binding, excision, integration, and host interactions. PiggyBac transposase is a pivotal tool in genetic research, facilitating DNA movement within and between genomes.

2020年7月10日 · We present cryo-EM structures of piggyBac transpososomes: a synaptic complex with hairpin DNA intermediates and a strand transfer complex capturing the integration step.

Given its flexibility for molecular engineering and its relatively high transposition activity as demonstrated in this study, piggyBac is a promising nonviral vector for mammalian transgenesis, with possibilities for targeted integration. piggyBac, …

For transgenesis that’s easy, consistent, and not limited by cargo size, SBI’s PiggyBac Transposon System is an excellent choice. The system consists of a PiggyBac Vector and the Super PiggyBac Transposase which recognizes transposon-specific inverted terminal repeats (ITRs) and efficiently integrates the ITRs and intervening DNA into the ...

19 小时之前 · Researchers in Japan have successfully used a nonviral piggyBac transposon system to introduce transgenes into cynomolgus monkeys, overcoming limitations of traditional virus-based methods. This breakthrough enables more precise and efficient genetic modifications in primates, opening new ...

1 天前 · Researchers successfully use a nonviral, piggyBac transposon system to introduce a transgene into cynomolgus monkeys.

2013年6月13日 · Here, we describe modified versions of piggyBac transposase that have potentially wide-ranging applications, such as reversible transgenesis and modified targeting of insertions. piggyBac is distinguished by its ability to excise precisely, restoring the donor site to its pretransposon state.

4 天之前 · Although lentiviral methods exist for generating transgenic monkeys, technical limitations hinder their utility. Here, the authors demonstrate that co-injecting piggyBac components with sperm into ...

Transposons are mobile genetic elements in the genome. The piggyBac (PB) transposon system is increasingly being used for stem cell research due to its high transposition efficiency and seamless excision capacity.