2021年9月17日 · Here, we describe a protocol for PI-PLC treatment of GPI-anchored proteins on intact cells for detection using flow cytometry. In the following protocols, cells were treated with …

磷酸肌醇特异性磷脂酶 C (PLC) 在跨膜信号转导中扮演重要角色。 响应细胞外刺激，如激素、生长因子和神经递质刺激后，PLC 水解磷脂酰肌醇-4,5 二磷酸 (PIP2) 并产生第二信使肌糖 1,4,5 …

摘要：旨在研究大肠杆菌产磷脂酰肌醇特异性磷脂酶C（PI-PLC）的发酵表达和分离纯化，探究PI-PLC酶切GPI锚定蛋白的效果。 依据NCBI数据库中蜡样芽孢杆菌的PI-PLC的基因序列，按照 …

2016年12月1日 · Phospholipase C (PLC) is an important enzyme of signal transduction pathways by generation of second messengers from membrane lipids. PLCs are also indicated to cleave …

2021年10月10日 · The glycosylphosphatidylinositol (GPI)-anchor modification attaches a lipid anchor to the C-terminus of a protein, tethering the protein to the cell surface membrane. From …

2009年6月5日 · Bloodstream forms of Trypanosoma brucei contain a glycosylphosphatidylinositol-specific phospholipase C (GPI-PLC) that cleaves the GPI-anchor of the variable surface …

摘要： 旨在研究大肠杆菌产磷脂酰肌醇特异性磷脂酶C（PI-PLC）的发酵表达和分离纯化,探究PI-PLC酶切GPI锚定蛋白的效果。 依据NCBI数据库中蜡样芽孢杆菌的PI-PLC的基因序列,按照大 …

Phosphatidylinositol-specific phospholipase C (PI-PLC) from Bacillus cereus (EC 3.1.4.10) cleaves the phospholipid phos- phatidylinositol (PI) into two molecules: water-soluble myo- …

2009年2月1日 · GPI-PLC (glycosylphosphatidylinositol-specific phospholipase C) is expressed in bloodstream-form Trypanosoma brucei, a protozoan that causes human African …

2002年1月29日 · Release of glycosylphosphatidylinositol- (GPI-) anchored ectoenzymes from the membrane by phosphatidylinositol- (PI-) specific phospholipases may play an important role in …