Biomark™ HD is an automated, high-performance PCR system that uses microfluidics technology to process samples at nanoliter-scale volumes for gene expression, genotyping, sample …
Automation and miniaturization of high-throughput qPCR for …
Quantitative PCR (qPCR) is a technique commonly employed in laboratories and core facilities. In our previous study, we had shown the possibility to automate steps in a panel-specific gene …
Performance of the Biomark HD real-time qPCR System (Fluidigm …
2019年4月24日 · We aimed to develop a novel nanofluidic real-time PCR (Fluidigm) assay that simultaneously detected and quantified 11 bacterial pathogens in 96 different samples (92 …
qPCR 分析、qPCR 仪器的工作原理及 qPCR 实验方案 - 知乎
qPCR，全称Quantitative Polymerase Chain Reaction，即定量聚合酶链式反应，是一种常见且较为重要的实验室技术，可以对目标DNA或RNA进行定性以及定量分析。 与传统的PCR不 …
We aimed to develop a novel nanofluidic real-time PCR (Fluidigm) assay that simultaneously detected and quantified 11 bacterial pathogens in 96 diferent samples (92 samples and 4 …
qPCR技术介绍（一）——基础概念 - Takara
2023年3月30日 · Real Time PCR，即实时荧光定量PCR，也被称为定量PCR或qPCR，是实时监测PCR扩增产物并进行解析的方法。 由于定量PCR具有操作简单、快速方便、灵敏度高、重 …
一文搞懂 PCR、qPCR、RT-PCR、RT-qPCR 及优缺点 - 知乎
pcr、qpcr、rt-pcr和rt-qpcr这四种技术的主要区别在于它们的应用领域和检测目标不同。 PCR主要用于DNA序列的扩增和检测；qPCR可以实时定量检测DNA或cDNA的含量；RT-PCR主要用 …
High Throughput PCR/qPCR (Fluidigm/Standard BioTools BioMark HD …
The BioMark HD is an automated, high-performance PCR/qPCR system that uses microfluidics technology to process samples at nanoliter-scale volumes for gene expression, genotyping, …
Microfluidic qPCR - High Throughput qPCR Core Facility of the ENS
The high-throughput quantitative PCR platform is equipped with Fluidigm’s Biomark-HD™, a system that allows the simultaneous quantification of the expression of tens of genes in a large …
qPCR-HD-GPC: A new core facility for high-throughput qPCR
2016年6月2日 · High throughput qPCR platform raises several questions in terms of sensitivity (minimal input), absolute quantification and data processing. To address these questions, we …