2017年11月9日 · Real-Time qRT-PCR (Real-Time Quantitative Reverse Transcription PCR) is a major development of PCR technology that enables reliable detection and measurement of products generated during each cycle of PCR process.

Quantitative reverse transcription polymerase chain reaction, also called RT-qPCR, is used to detect and quantify RNA. Total RNA or mRNA is first transcribed into complementary DNA (cDNA). The cDNA is then used as the template for the quantitative PCR or real-time PCR reaction (qPCR).

Guidance documents issued from numerous international regulatory authorities recommend the use of quantitative polymerase chain reaction (qPCR) and/or quantitative reverse transcriptase PCR (qRT-PCR) assays due to their highly sensitive and robust target-specific detection.

2020年6月15日 · According to MIQE, the acronym ‘qPCR’ describes quantitative real-time PCR, which is the PCR amplification of DNA in real time, measured by a fluorescent probe, most commonly an intercalating dye or a hydrolysis-based probe, enabling quantitation of the PCR product (see Figure 1B).

Real-time PCR can be used for both qualitative and quantitative analysis; choosing the best method for your application requires a broad knowledge of this technology. This section provides an overview of real-time PCR, reverse-transcription quantitative PCR techniques, and the choice of instruments that Bio-Rad offers for these techniques.

Real-Time qRT-PCR (Real Time q uantitative R everse T ranscription PCR) is a major development of PCR technology that enables reliable detection and measurement of products generated during each cycle of PCR process.

Quantitative real-time reverse-transcription PCR (qRT-PCR) is the most common approach for gene expression quantification and one of the most common methods used to detect low levels of miRNA with high sensitivity. qRT-PCR is a sensitive and specific method for miRNA expression measurement that can be used for absolute quantification.

qRT-PCR is a quantitative variant of typical PCR. It is used to determine how much of a particular DNA sequence is present in a mixture. This can be used to determine gene expression levels (following RT-PCR of cellular RNAs), gene copy number, and for other applications.

qRT-PCR (real-time reverse transcription-PCR) has become the benchmark for the detection and quantification of RNA targets and is being utilized increasingly in novel clinical diagnostic assays.

RT-qPCR enables quantitative measurement of the levels of mRNAs, small RNAs, and other noncoding RNAs (Chen et al., 2011; Ding et al., 2022, Kristensen et al., 2019). The accuracy and efficiency of RT-qPCR depend on the choice of reagents and the design of …