2018年5月29日 · Here, we developed a simplified, rapid FISH technique using pre-labeled oligonucleotide probes (PLOPs) and tested the procedure using 18 PLOPs from 45S and 5S rDNA, Arabidopsis -type telomere,...

2022年3月24日 · 荧光原位杂交（Fluorescence in situ hybridization FISH）是一门新兴的分子细胞遗传学技术，是 20 世纪 80 年代末期在原有的放射性原位杂交技术的基础上发展起来的一种非放射性原位杂交技术。

荧光原位杂交 (fluorescence in situ hybridization，FISH)是一种利用荧光标记的核酸片段为探针，与组织、细胞或染色体上待测DNA或RNA互补配对，结合成专一的核酸杂交分子，通过荧光检测系统将待测核酸在组织、细胞或染色体中的位置显示出来的技术。

2021年11月24日 · 常规石蜡包埋组织切片（常规切片）是组织学常规制片技术中最为广泛应用的方法，在病理学中常用以研究、观察及判断细胞组织的形态变化，与免疫学技术结合可用于组织切片中细胞组织的多肽及蛋白质等大分子物质的定性和定位观察研究，与FISH方法结合可 ...

2023年3月1日 · With 18S rDNA as a probe, 45S rDNA repeat units were successfully visualized on the BAC 625-E18 molecular fiber by FISH. FISH results illustrated that the hybridization signal points representing repeat units were lined like continuous …

2020年9月1日 · In this study, we isolated and cloned 5S rDNA sequences from various toxin-producing species of the genus Alexandrium and developed a fluorescence in situ hybridization (FISH) probe that allows their chromosomal localization.

2020年4月5日 · The localization of ribosomal DNA (rDNA) using fluorescence in situ hybridization (FISH) has been widely used to study genetic relationships among various plant species (Abirached-Darmency et al., 2005; Cerbah et al., 1998; Hanson et al., 1996; Hasterok et al., 2001; Robledo et al., 2009; Zoldos et al., 1999).

2015年12月3日 · We revealed that 5S rDNA signal can be applied to discern A-genome from B-genome, and that 5S rDNA bearing chromosomes can be stably passed down in successive generations. Our work provided a significant method in fish breeding and this is important for studies in fish evolutionary biology.

2002年5月3日 · Single-colour FISH consistently mapped one chromosome pair per spread using either 18S or 5S rDNA as probes. The telomeric sequence (TTAGGG)n hybridized with termini of all chromosomes whereas...

2013年6月27日 · In this study, we used chromosome-specific BAC clones from G. hirsutum, 5S and 45S rDNA as multiple probes to have FISH located precisely the 5S and 45S rDNA of tetraploid species and species of their donor genome (A and D genome).