scFv-GCN4-DNMT3a-DNMT3l - Addgene
Expresses the scFv-GCN4-DNMT3a-DNMT3l fusion protein (more details are shown in the vectro map) for targeted DNA methylation. Should be used in a combination with the dCas9-SunTag …
A Protein-Tagging System for Signal Amplification in Gene Expression ...
2014年10月23日 · Importantly, fluorescence imaging revealed that the scFv-GCN4 antibody robustly bound to the GCN4 v4 peptide array in vivo and FRAP analysis suggests that the …
pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS Sequence and Map
pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS Vector encoding a single-chain anti-GCN4 peptide antibody fused to superfolder GFP, the VP64 activation domain, and the GB1 …
pHR-scFv-GCN4-sfGFP-GB1-dWPRE 载体图谱和序列 - 纽普生物
提供 pHR-scFv-GCN4-sfGFP-GB1-dWPRE 载体/质粒图谱，全长序列，抗性，大小和元件信息。
1P4I: Crystal Structure of scFv against peptide GCN4 - RCSB PDB
2003年4月23日 · We generated a single chain Fv fragment of an antibody (scFv) with a binding affinity of about 5 pm to a short peptide by applying rigorous directed evolution. Starting from a …
Antibodies 101: Single Chain Fragment Variables (scFvs) - Addgene
2021年6月3日 · The SunTag system uses an scFv to amplify the fluorescence intensity of a tagged protein. SunTag has two components: 1) a protein of interest that’s tagged with 10-24 …
Targeted DNA demethylation in vivo using dCas9–peptide repeat and scFv ...
2016年8月29日 · To achieve efficient recruitment of an anti-GCN4 scFv fused to the ten-eleven (TET) 1 hydroxylase, which induces demethylation, we changed the linker length to 22 amino …
SunTag-PE: a modular prime editing system enables versatile and ...
2025年3月18日 · The first was the enrichment effect of GCN4-scFv, which was verified by the higher PE editing efficiency of the functional SunTag-PE system compared to the GCN4-scFv …
pHR-scFv-GCN4-mStayGold-GB1-dWPRE - Addgene
Plasmid pHR-scFv-GCN4-mStayGold-GB1-dWPRE from Dr. Marvin Tanenbaum's lab contains the insert scFv-GCN4-mStayGold and is published in Cell. 2025 Jan 23:S0092-8674(25)00045 …
CRISPR/dCas9/gRNA活细胞基因组标记系统的比较及优化 - 新闻 …
2018年3月30日 · 为了增加标记信号，研究人员将 24 次重复的 GCN4 多肽添加到 dCas9 的 C 端，同时将荧光蛋白与特异性识别 GCN4 的单链抗体 scFv 融合表达，通过这种方式可以将更多 …