2022年11月4日 · Detailed characterization of treated tissue or cell samples is required for validation of the therapeutic and is performed using immunohistochemistry (IHC) or ImmunoFluorescence (IF). To ensure the successful detection of scFvs, it is important to use the correct antibodies which recognize the species and sequence used to generate them.

2021年8月25日 · A small-sized scFv fragment can target colon cancer secreted protein-2 (CCSP-2), highly expressed in colorectal adenocarcinoma tissues; moreover, its full-length IgG probe has been used for ...

2018年10月9日 · Single chain fragment variable (scFv)-phages issuing from a human combinatorial library were selected on the lesions induced in a rabbit model of atherosclerosis after three rounds of...

ScFv，全称为“Single-Chain Fragment Variable”，中文翻译为“单链可变区片段”，是一种基因工程融合蛋白 [1]，是由抗体重链的可变区与轻链的可变区在一段肽链（linker，后文有详细介绍）的连接下构成的小分子（图1） [2]。 图1：抗体模型：亚基的组成以及沿多肽链的domain分布，ScFv抗体由抗体重组技术得到。 Fv片段是具有抗原结合活性的最小的免疫球蛋白分子单位。 抗体片段（antibody fragment）也可与一系列小分子相融合（fuse） [3]，包括用于癌症成像的放射性同 …

scFv 和VHH 具有分子质量小、穿透力强、制备周期短、结构简单、易于工程化改造等优点，在免疫治疗、 影像诊断、药物递送、病毒感染、生物监测、生物传感器、基础研究工具等领域被广泛使用。

2024年4月8日 · The results showed that we provided a stable method to display EGFR-specific scFv on the surface of exosomes. Exo scFv exhibited accelerated BBB penetration and prolonged tumor accumulation in BM lesions compared to exo ctrl, indicating increased gene silencing efficacy and diminished adverse effects. However, xenograte tumors in brain were ...

To date, generation of single-chain fragment variable (scFv) has become an established technique used to produce a completely functional antigen-binding fragment in bacterial systems. The advances in antibody engineering have now facilitated a more efficient and generally applicable method to produce Fv fragments.

2018年3月7日 · In the present study, an scFv antibody named scFv-A8 with high specificity was obtained by phage display and biopanning, with the affinity 2.6 × 10 9 L/mol. Maltose binding protein (MBP) was used to improve the solubility of scFv by inserting an linker DNA between scFv and MBP tag, and the resulted fusion protein (MBP-LK-scFv) has high ...

基于嵌合抗原受体 (CAR) 的细胞疗法的开发正在彻底改变人类癌症和自身免疫性疾病的治疗。 随着这种创新的治疗方式不断改进并变得更加容易获得，更好的试剂正在提高我们检测、识别和充分检测 CAR 表达细胞的能力。 凭借不断增长的创新产品组合，CST 正在帮助推进该领域研究人员的工作。 科学家在开发和检测 CAR 工程化细胞时面临的最大挑战之一是需要强大、灵敏且有选择性的研究试剂来识别 CAR 工程化细胞。 如果您计划在研究中使用免疫检测，那么开发自己的抗 …

To date, generation of single-chain fragment variable (scFv) has become an established technique used to produce a completely functional antigen-binding fragment in bacterial systems. The advances in antibody engineering have now facilitated a more efficient and generally applicable method to produce Fv fragments.