We characterized the loss-of-function mutants alg‑1 (gk214) and alg‑2 (ok304), which lack the two Argonaute-family members important for microRNA function, by using different proteotoxic stress assays (Grishok et al. 2001).

2020年6月2日 · Whole-genome resequencing revealed a mutation in asparagine-linked glycosylation 12 that encodes the mannosyltransferase responsible for adding the eighth mannose residue in an α-1,6 linkage to the dolichol-PP-oligosaccharide N …

A cytoplasmic reporter, RFP, and a nucleic reporter, Histone::YFP (hYFP), were used to label the somas and nuclei, respectively, when the membrane reporter was insufficient to identify the GAL4...

2019年4月30日 · Here we provide evidence that ANXA7 upon plasma membrane injury is recruited to the site of injury where it forms a complex with the Ca 2+ -binding protein apoptosis-linked gene-2 (ALG-2). We...

2020年7月20日 · Alix (ALG-2 interacting protein X)/ PDCD6IP) is an adaptor 51 protein binding to ESCRT and endophilin-A proteins and thereby driving deformation and 52 fission of endosomal and cell surface membranes.

2014年12月23日 · These results demonstrate Ca 2+ -dependent accumulation of ESCRT III-Vps4 complex following large focal injury to the cell membrane and identify the role of ALG-2 as the initiator of sequential...

2021年10月14日 · During larval development in Caenorhabditis elegans, Argonaute-Like Gene 1 (ALG-1) is the primary mediator of the miRNA pathway, while the related ALG-2 protein is largely dispensable.

... give rise to adult ALG and EG To examine the fate of the larval neuropil-glia, we first traced larval ALG in the central brain using Histone::YFP, which localizes in the nuclei and allows...

2022年11月6日 · 我们知道黄色荧光蛋白YFP的激发波长为300-520nm，主峰在505nm；绿色荧光蛋白GFP的激发波长为300-510，主峰在488nm，如果用绿色荧光蛋白GFP 488nm的激发光源激发，黄色荧光蛋白YFP也是能够吸收足够的能量被激发，但是效果没有505nm的激发光源激发后的效 …

通常是使用激发/发射光谱有重叠的两个荧光蛋白。目前蛋白-蛋白相互作用研究中最广泛应用的FRET对就是青色荧光蛋白（cyan fluorescent protein, CFP）、黄色荧光蛋白（yellow fluorescent protein, YFP）。