分子克隆——ccdB（有效克隆的关键毒性基因） - 知乎
ccdB基因位于大肠杆菌的 F质粒 上，是被ccd操纵子编码的毒性-抗毒性系统的一部分，确保含F质粒的细胞分裂后F质粒仍存在（分裂后含F质粒的细胞才能存活）。 ccdB编码的毒性蛋白 …
RecET direct cloning and Redαβ recombineering of ... - Nature
2012年4月29日 · The BAC vector is generated by inserting into pBAC2015 a ccdB gene together with an ampicillin resistance gene and a pBR322 replication origin flanked by homology arms …
Direct cloning of a herpesvirus genome for rapid generation of ...
2023年1月1日 · In this study, we demonstrated a streamlined approach for creating infectious BAC clones of PRV by ExoCET. The linear BAC vector flanking the homologous sequences …
Improved seamless mutagenesis by recombineering using ccdB …
2013年12月24日 · Seamless BAC mutagenesis using ccdB counterselection. A functional test based on β-galactosidase expression from pBeloBAC11 was used to evaluate the efficiency …
Plant Gene Modification by BAC Recombineering | SpringerLink
2022年5月19日 · Here we describe two recombineering protocols for modification and transfer of plant genes from BACs into Agrobacterium T-DNA plant transformation vectors. The first …
Gene modification by fast‐track recombineering for cellular ...
A PCR‐based BAC‐recombineering protocol accelerating site‐specific modification of plant genes with suitable tags is described and exploited for purification, cellular localization, …
Seamless site-directed mutagenesis in complex cloned DNA
2024年7月15日 · By combining recombineering, ccdB counterselection and exonuclease-mediated in vitro annealing, RedEx achieves seamless mutagenesis of large DNA molecules, …
【科普】ccdB筛选 - 知乎 - 知乎专栏
Gateway 的核心之一就是利用 ccdB 的毒性，从而达到negative selection. ccdB是一种DNA gyrase， 其工作原理如下图： 它能让DNA的超螺旋结构变成逆向螺旋，因此对 E.coli 具有毒性 …
BAC‐recombineering for studying plant gene regulation: developmental ...
2010年12月13日 · Recombineering, permitting precise modification of genes within bacterial artificial chromosomes (BACs) through homologous recombination mediated by lambda phage …
Markerless bacterial artificial chromosome manipulation method …
2023年8月1日 · The ccdB gene, which codes for the toxic protein CcdB, is commonly used for counter-selection systems, including BAC recombineering [11]. Expression of CcdB is toxic to …