Explore Bio-Rad’s comprehensive pGLO and GFP teaching resources, including pGLO plasmid maps, hands-on lab activities, and free presentations, videos, and case studies. Learn how to use GFP to illuminate gene regulation and expression in your classroom.

Bacterial transformation plasmid for regulated expression of GFP. Sequence Author: Bio-Rad. Explore Over 2.7k Plasmids: Fluorescent Protein Genes & Plasmids | More Plasmid Sets. Efficient cleavage requires at least two copies of the SgrAI recognition sequence. Sticky ends from different BsmBI sites may not be compatible.

The pGLO plasmid contains an origin or replication, a selectable marker, and the gene for Green Fluorescent Protein (GFP). The plasmid also contains a gene for the arabinose C protein, which is a protein that regulates expression from the arabinose BAD promoter (P BAD).

Plasmid pGLO-GFP-1UAG from Dr. Chang Liu's lab contains the insert Green Fluorescent Protein with 1 UAG codon and is published in Integr Biol (Camb). 2016 Apr 18;8(4):542-5. doi: 10.1039/c5ib00232j. Epub 2015 Nov 27.

This application note describes how the green fluorescent protein (GFP) expressed from Bio-Rad’s pGLO plasmid can be used to help illustrate and teach the central dogma of biology, from the transformation of DNA to the expression of a protein to the visualization of a trait.

BIO-RAD pGLO Plasmid Map and Sequence Plasmid Map The features of the pGLO plasmid are illustrated in the plasmid map below. Specific sequences, including gene and promoter sequences, are shown as thick lines along the circle with arrowheads that point in the direction of operation or transcription. Short lines

2024年9月6日 · In the map of the pGLO plasmid the 239 amino acid open reading frame (ORF) is green fluorescent protein (GFP). If you were working in the lab and wanted to clone the GFP into a different plasmid, how would you go about removing the GFP from the pGLO plasmid.

2024年9月5日 · In the map of the pGLO plasmid the 239 amino acid open reading frame (ORF) is green fluorescent protein (GFP). If you were working in the lab and wanted to clone the GFP into a different plasmid, how would you go about removing the GFP from the pGLO plasmid.

Cells which were treated with DNA (+pGLO) should contain the pGLO plasmid and should express the ampicillin resistance gene—the corresponding LB/amp plate will contain transformed bacterial colonies.

pGLO contains several DNA sequences that enable replication of the plasmid DNA and expression of the fluorescent trait (phenotype) in bacteria following transformation. The essential sequences include the following: GFP— the jellyfish gene that codes for the production of green fluorescent protein