Human CD28 qPCR Primer Pair，即人CD28 qPCR引物对，主要用于基于SYBR Green的qPCR、One-Step qRT-PCR或semi-quantitative PCR。 本引物为预先设计、经过qPCR验证、预混的引物对。

A real-time quantitative reverse-transcription polymerase chain reaction (RT-PCR) assay for CD28 mRNA was carried out with a QuantStudio™ 12K Flex real-time PCR system (Thermo Fisher Scientific) using GeneAce SYBR ® qPCR Mix α (NIPPON GENE Co., Ltd.) according to the manufacturer’s instructions.

2025年1月14日 · In this study, we develop a DDMP evolving system based on yeast display and error-prone PCR to identify high-affinity peptide binders. We obtained DDMPs with a picomolar affinity for CD28, exceptional binding specificity, and remarkable oxidative folding efficiency.

2012年5月15日 · CD28 is one of the most important co-stimulatory molecules required for effective activation of resting T cells in human and mouse. However, there are few studies on porcine CD28 (pCD28) until now. In the present study, we cloned and characterized the full-length cDNA of CD28 from the miniature pig.

2002年1月1日 · Reverse-transcriptase-polymerase chain reaction (RT-PCR) analysis of CD28 mRNA in human T cells demonstrating the presence of eight different mRNA isoforms. In the 5% polyacrylamide gel: lanes 1 and 3 = 100 bp DNA molecular size ladder; lane 2 = primer combination f1 + r1; and lane 4 = primer combination f1 + r2.

抗 CD3（小鼠和人）的最佳浓度范围为 1-3 µg/mL；抗 CD28（小鼠和人）的最佳浓度范围为 3-5 µg/mL。 方案提示： IL-2 会促进细胞毒性 T 细胞的生长和分化。

TaqMan™ Gene Expression Fusion Assays are designed to detect fusion transcripts using real-time PCR. Our collection covers gene fusion transcripts from sources including NCBI Genbank and content on Oncomine™ products.

Real-time PCR primer assay designed for SYBR ® Green gene expression analysis. Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

CD28, described as a T cell costimulatory molecule so far, is expressed on human peripheral blood neutrophils, as shown by cell surface staining and immunoprecipitation with anti-CD28 monoclonal antibody, and by reverse transcription PCR. The phorbol 12-myristate 13-acetate-augmented expression of C …

PrimePCR™ PreAmp for Probe Assay: Cd28, Mouse Reaction: 400 reactions Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.