2018年7月2日 · Long fibers forming Velcro-like structures in contact sites between the cells were identified in colony biofilms, but not in smooth colonies of the BR-F-flo11 strain, by transmission electron microscopy (TEM) of chemically fixed cells .

1988年12月15日 · The gene was further localized to 3.5 kb by mapping of the CYC8 mRNA and insertional mutagenesis. Insertion and deletion mutations are viable and produce the same array of phenotypes as point mutations. CYC8 disruptions also had effects on the mating ability and morphology of MATα cells similar to that of tup1 mutations.

2011年12月1日 · The yeast Tup1-Cyc8 corepressor complex is recruited to promoters by DNA-binding repressors, but the mechanisms by which it inhibits expression of genes involved in various stress pathways are poorly understood. Conditional and rapid depletion of Tup1 from the nucleus leads to concurrent nucleosome …

2023年8月11日 · We examined TUP1 and CYC8 single and double gene deletion mutants and show that the Tup1 and Cyc8 proteins can make distinct contributions to the regulation of Tup1-Cyc8 target genes. Furthermore, we show that Tup1p and Cyc8p can be found independently of each other to negatively regulate gene transcription and can persist at active genes to ...

2016年1月22日 · We propose that sumoylation protects against persistent inclusion formation during hyperosmotic stress, allowing optimal transcriptional function of the Cyc8-Tup1 complex. Cells have evolved complex stress responses to cope with environmental challenges that could otherwise inflict severe damage on the molecules essential for life.

1994年6月30日 · Here we show that when it is bound upstream of a functional promoter through the Lex A DNA-binding domain, Tupl represses transcription in the absence of Cyc8.

1999年1月1日 · Cyc8 (Ssn6)-Tup1, a general co-repressor complex, is recruited to promoter DNA via interactions with DNA-binding regulatory proteins and inhibits the transcription of many different yeast genes.

2018年7月2日 · We have identified here antagonistic functional interactions between transcriptional regulators Cyc8p and Tup1p that modulate the life-style of natural S. cerevisiae strains between biofilm and domesticated mode.

2023年8月11日 · We examined TUP1 and CYC8 single and double deletion mutants and show that CYC8 represses more genes than TUP1, and that there are genes subject to (i) unique repression by TUP1 or CYC8, (ii) redundant repression by TUP1 and CYC8, and (iii) there are genes at which de-repression in a cyc8 mutant is dependent upon TUP1, and vice-versa.

2018年7月2日 · Our data show that Tup1p and Cyc8p regulate biofilm formation in the opposite manner, being positive and negative regulators of colony complexity, cell-cell interaction and adhesion to surfaces.