
表观遗传学/ChIP-Seq实验中标签抗体怎么选 - 知乎专栏
gfp(绿色萤光蛋白),是一个由约238个氨基酸组成的蛋白质,从蓝光到紫外线都能使其激发,发出绿色萤光。 GFP标签可位于蛋白质的C端或N端。
GFP抗体(ab290)概述| Abcam中文官网
gfp 抗体可以放大较弱的 gfp 信号,或进行ip实验。 我们的兔多克隆 GFP 抗体(ab290) 经过流式细胞术、IHC、ICC、IP、和 WB 应用的测试,且适用于 ChIP。 图注: 来自客户的 GFP 抗体(ab290)实验数据。
Anti-GFP antibody - bioluminescent marker (ab290) - Abcam
Anti-GFP antibody is a rabbit polyclonal antibody that is used in green fluorescent protein (GFP) western blot (WB), immunocytochemistry/immunofluorescence (ICC/IF), electron microscopy (EM), enzyme-linked immunosorbent assay (ELISA), immunohistochemistry - PFA perfusion fixed frozen sections (IHC-FoFr), immunohistochemistry on paraffin ...
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碧云天生物技术-GFP抗体(小鼠单抗)(AG281) - Beyotime
本GFP抗体(小鼠单抗),即mouse monoclonal GFP antibody,为进口分装,用全长的来源于水母的GFP (green fluorescent protein)作为抗原制备得到的抗GFP小鼠单克隆抗体。克隆号为B-2。 本GFP抗体可以识别GFP以及GFP的一些突变体例如EGFP(enhanced green fluorescent protein)。
绿色荧光蛋白 (GFP) - Abcam中文官网
绿色荧光蛋白 (GFP) 是一种 β-桶状蛋白 1 ,由 238 个氨基酸组成,分子量约为 27 kDa。 GFP 是从水晶水母 Aequorea victoria 分离而来。 GFP 可通过能量转移,将水 母发光蛋白通过化学作用发出的蓝色荧光转变为绿色荧光 2 。
GFP 抗体 | Thermo Fisher Scientific - CN
绿色荧光蛋白 (GFP) 是一种 27 kDa 单体蛋白,从 维多利亚水母 中分离出来,可自动催化形成荧光色素。 GFP 是监测生理过程、可视化蛋白定位和检测转基因表达的通用标记物。 GFP 也是评估固定细胞或活细胞中基因表达和亚细胞蛋白分布的强大研究工具。 GFP 在暴露于紫外或蓝光 (395nm) 时,以稳定且非物种依赖性的方式发出明亮的绿色荧光(峰值发射 508nm),因此非常适合作为标记物用于荧光显微镜、细胞分析、标记融合蛋白以及检测体内基因启动子的转录调控 …
经 ChIP-seq 验证的抗体 | Cell Signaling Technology
ChIP-seq 是一种强大的技术,可结合 ChIP 和下一代测序分析 (NGS) 来研究整个基因组中的 DNA-蛋白质相互作用,但它实际上只是与在 ChIP 实验中使用的抗体的性质差不多。
标签/内参抗体 -GFP Antibody - Abmart
The green fluorescent protein (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequorea victoria. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors, making GFP a useful tool for monitoring gene expression and protein ...
Anti-GFP antibody - ChIP Grade | Abcam ab290 product information
2022年8月23日 · Migration and Differentiation of GFP-transplanted Bone Marrow-derived Cells into Experimentally Induced Periodontal Polyp in Mice. Int J Med Sci. 2016;13:500-6 pubmed publisher
重组Anti-GFP抗体[RM1064] (ab316291) - Abcam中文官网
购买GFP兔重组Multiclonal [RM1064](ab316291),GFP抗体经WB,IP,IHC-P,ChIP,I-ELISA验证,可与无种属依赖性样本反应。中国现货速达。
GFP Antibody - Cell Signaling Technology
GFP Antibody detects GFP, YFP, and CFP-tagged proteins exogenously expressed in cells. This antibody does not detect RFP-tagged proteins. Please note that the GFP, YFP, and CFP tags add approximately 27 kDa to the molecular weight of the fusion protein.
GFP抗体 (GTX113617) | GeneTex中文官方网站
GFP antibody recognizes the green fluorescent protein (GFP), a fluorophore in widespread use in biomedical research. GFP fluorescence allows real-time study of physiological processes involving fusion proteins or GFP-expressing cells. This antibody also detects other GFP variants, including EGFP and YFP, but not red fluorescent protein (RFP).
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Anti-GFP antibody [RM1064] (ab316291) - Abcam
Rabbit Recombinant Multiclonal GFP antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra), IP, ChIP, I-ELISA and reacts with Tag samples.
染色质免疫沉淀(ChIP)技术笔记之(6):操作步骤Protocol_chip质控-…
2024年8月28日 · 染色质免疫沉淀法 (chip) 是一种基于抗体的技术,可用来选择性地使特异性 dna 结合蛋白及其 dna 靶标富集。chip 可用来研究某种特殊的蛋白-dna 相互作用、多种蛋白-dna 相互作用或全基因组或部分基因内的相互作用。
Anti-GFP Antibody Products - Biocompare
Anti-GFP antibodies are useful reagents for the immunodetection of GFP (green fluorescent protein), a commonly used epitope tag for recombinant proteins in applications such as immunofluorescence, flow cytometry, or western blot.
Anti-GFP tag Rabbit pAb - ChIP, CUT&RUN and CUT&Tag Grade
Rabbit monoclonal Anti-GFP tag antibody - ChIP Grade, Catalog # AT0044 - Epitope Tag Antibody. Validated in WB, IHC, IF, IP, ChIP applications and tested in E.coli, yeast, Sf9, CHO, HEK293F expression
Anti-GFP Antibodies | Thermo Fisher Scientific - US
Our anti-GFP antibodies are highly specific; react only with the intended target and in so doing contribute to low background signal and increased signal to noise ratio. All our anti-GFP antibody conjugates are suited for detection of native GFP, GFP variants, and most GFP fusion proteins by western blot analysis and immunocytochemistry.
一文分清IP、co-IP、ChIP、RIP、pull-down! - 知乎专栏
染色质免疫共沉淀技术(Chromatin Immunoprecipitation,ChIP)是用来研究蛋白质与DNA是否在体内存在相互作用,常用来检测转录因子结合位点。 利用抗原抗体的特异性结合,将与目的蛋白相结合的DNA片段沉淀下来,能够真实地反映结合在DNA序列上的调控蛋白。
Genome-wide mapping of endogenous G-quadruplex DNA …
2018年2月22日 · This protocol adapts traditional ChIP-seq for the detection of DNA secondary structures through the use of a G4-structure-specific single-chain antibody with refinements in chromatin ...
Good anti-GFP/YFP for ChIP-Seq? - SEQanswers
2012年1月27日 · Just wondering if anyone could recommend a good anti-GFP/YFP for ChIP-Seq. I've tried quite a few (including ab290, JL-8) for ChIP-qPCR. I just found the polyclonal gives rather high background, and the monoclonal seems to have low efficiency (I'm working on Arabidopsis). Also, for ChIP-Seq, is monoclonal Ab a better choice over polyclonal?