The green fluorescent protein (GFP) is a protein that exhibits green fluorescence when exposed to light in the blue to ultraviolet range. [2][3] The label GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria and is sometimes called avGFP.

Invitrogen Green Fluorescent Protein (GFP) is a useful expression label in the blue channel for flow cytometry and imaging applications. GFP can be excited by the 488 nm laser line and is optimally detected at 510 nm.

If you are considering creating a fluorescent fusion protein to use in fluorescence imaging, GFP—or more specifically, the enhanced version of the original Aequorea victoria GFP called enhanced GFP or EGFP—is your best choice. It’s monomeric, bright, well behaved, noninvasive, and virtually indestructible.

2016年2月9日 · Specific cell surface labeling is essential for visualizing the internalization processes of G-protein coupled receptors (GPCRs) and for gaining mechanistic insight...

Ready to use in just 90 minutes, our protein labeling kits include an easy-to-use, pre-packed spin column for rapid dye removal and typical recovery greater than 85%. Each kit contains enough reagent for 3–5 protein conjugation reactions.

2011年4月4日 · The objectives of this study were to label major foodborne pathogens and related bacteria, including Listeria monocytogenes, Listeria innocua, Salmonella, and Escherichia coli O157:H7 strains, with GFP and characterize the labeled strains for stability of the GFP plasmid and the plasmid's effect on bacterial growth.

2021年4月10日 · GFP绿色荧光蛋白是一种基本的（构成荧光的）长斯托克斯位移荧光蛋白，发表于1992年，来源于Aequorea victoria。它对酸的敏感性低。GFP激发光的波长是395nm，mEGFP发射光的波长是509nm。 图一：wtGFP， avGFP荧光蛋白的激发光和发射光. EGFP又名增强型GFP，GFPmut1

Read on to learn more about GFP, how scientists have evolved this versatile protein to suit their experimental needs, and some of the common applications in the lab. Why Green Fluorescent Protein? GFP is a ~27 kDa protein consisting of 238 amino acids derived from the crystal jellyfish Aequorea victoria .

2024年8月19日 · GFP-tagged tubulins have been widely used to monitor and visualize tubulin isotypes, but this is known to interfere with their cellular functions. This study describes a functional labeling strategy, guided by AlphaFold2 structural predictions, to label endogenous tubulins across diverse species while preserving functionality.

红色荧光蛋白(drFP583 )是人们从珊瑚虫Discosoma gen。中克隆的一种与绿色荧光蛋白(GFP)同源的荧光蛋白，在紫外光的照射下可发射红色荧光，有着广泛的应用前景;但它自身的缺点如寡聚化、成熟缓慢等限制了它的进一步应用。