To mimic the entrance of bacterial-derived compounds into the host, an endotoxemia model has been adopted in which lipopolysaccharide (LPS), a component of the outer cell wall of Gram-negative bacteria, were injected into mice.

脂多糖（Lipopolysaccharide, LPS）是革兰氏阴性细菌外膜的主要成分之一，由类脂A、核心多糖和O-抗原三部分组成，其中O-抗原决定了LPS的血清型，具有重要的生物学功能和作用机制。

2 天之前 · Acute systemic immune activation may affect gut-luminal colonisation dynamics. Here, the authors show that LPS exposure in mice elevates gut-luminal oxygen species levels, inhibiting the ...

2020年8月7日 · In this study, we evaluated the utility of an intracerebellar injection of lipopolysaccharide (LPS) to generate an animal model of inflammatory CA. We observed that LPS administration induced...

2021年3月1日 · Adult, male C57/B6 mice received lipopolysaccharide (LPS) injections (20 mg/kg). We recorded survival rates and metabolic changes, such as resting energy expenditure (REE), compared with controls up to 72 h after LPS administration.

1 天前 · LPS treatment enhances prion disease susceptibility when given 3 h before infection Groups of male and female mice were first given a single IP injection of LPS (10 µg) and subsequently injected ...

2024年10月8日 · We found that acute LPS administration (0.83 mg/Kg i.p.) reduced body weight, food intake, and glucose levels compared to the saline-injected mice, concomitant with decreased activity in home cage monitoring. Locomotion was significantly reduced in Open Field, Introduced Object, and Y-Maze tests.

2 天之前 · Here, we model acute, pathophysiological lipopolysaccharide concentrations in mice and show that systemic exposure promotes a 100-10'000-fold expansion of Klebsiella pneumoniae, Escherichia coli, Enterococcus faecium and Salmonella Typhimurium in the gut within one day, without overt enteropathy.

An intracellular recognition of LPS is provided by specialized inflammatory caspases, namely caspase 4 and 5 in humans, and caspase 11 in mice, and this signaling pathway is also being called a ‘non-canonical’ inflammasome activation [33].

2019年7月6日 · The presented protocol describes a detailed step-by-step procedure to induce ALI in mice by direct intratracheal LPS instillation and perform FACS analysis of blood samples, bronchoalveolar lavage (BAL) fluid, and lung tissue. After intraperitoneal sedation, the trachea is exposed and LPS is administered via a 22 G venous catheter.