Efficient feeder cells preparation system for large-scale …
2017年9月25日 · In SAM, mouse embryonic fibroblast (MEF) growth were fully inhibited by 10 μg/ml mitomycin-C (MMC) in 0.5 hours, and the feeder cells generated display higher …
Methanol fixed fibroblasts serve as feeder cells to maintain stem …
2018年5月17日 · Preparation of mouse embryonic fibroblast (MEF) feeder cells to maintain pluripotent stem cells (PSCs) is time consuming and involved in animal issues. Here, we …
Feeder cells treated with ethanol can be used to maintain …
2023年1月4日 · Human induced pluripotent stem cells were seeded on ethanol‐fixed MEF (ET‐MEF) cells, and mitomycin C‐treated HDF (MMC‐HDF) cells. The results showed that the …
饲养层细胞如何获得 - 知乎 - 知乎专栏
MEF细胞 的培养基. 培养基： DMEM-H +10% FBS +双抗. 二． MEF的解冻. 1. 从液氮中取出一支冻存的细胞，迅速将其投入37度水浴锅中，震荡，使其尽快溶解； 2. 迅速将冻存管转移至超 …
Feeder Cells Support the Culture of Induced Pluripotent Stem
2012年3月2日 · Mitomycin-C (MMC)-treated MEF feeder cells were prepared by incubating MEFs with DMEM medium supplemented with 10% FBS and 10 µg/mL of MMC at 37°C for 3–4 h. …
小鼠胚胎成纤维细胞的培养 - 知乎 - 知乎专栏
小鼠胚胎成纤维细胞(mef) 作为原代培养细胞，其刺激增殖的能力强且可靠、易培养、来源丰富，常被用作干细胞培养的饲养层。 一方面提供刺激干细胞增殖的各种因子，另一方面保持干 …
MEF为何要用mc处理？目的和作用是什么 - 实验室综合讨论区 干 …
2013年5月27日 · mmc作用不是灭活，只是抑制MEF的分裂而已，但MEF细胞仍然会长大变长。 若MEF不经过mmc处理，直接培养ips的话，得出的结果是MEF生长速度快于ips，最后MEF完全 …
Three key variables involved in feeder preparation for the …
2009年7月1日 · Mitomycin C (MMC) is used to inactivate mEFs, but this causes DNA damage, and it is unclear whether MMC remains in the culture system after several washes. Three …
Methanol fixed fibroblasts serve as feeder cells to maintain stem …
2018年5月17日 · Preparation of mouse embryonic fibroblast (MEF) feeder cells to maintain pluripotent stem cells (PSCs) is time consuming and involved in animal issues. Here, we …
Optimized CRISPR-Cas9-based Strategy for Complex Gene …
Treatment of MEFs with Mitomycin (MMC) MEF cell density for use as an MMC-treated feeder cell layer is 2.5 × 10 6 cells per 10 cm dish. Treat MEF cells with MMC (10 µg/mL in MEF medium) …